FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2296756579> ?p ?o ?g. }

• W2296756579 abstract "Abstract Otitis media (OM) is a common pediatric disease for which systemic antibiotics are often prescribed. While local treatment would avoid the systemic treatment side-effects, the tympanic membrane (TM) represents an impenetrable barrier unless surgically breached. We hypothesized that the TM might harbor innate biological mechanisms that could mediate trans-TM transport. We used two M13-bacteriophage display biopanning strategies to search for mediators of trans-TM transport. First, aliquots of linear phage library displaying 10 10th 12mer peptides were applied on the TM of rats with active bacterial OM. The middle ear (ME) contents were then harvested, amplified and the preparation re-applied for additional rounds. Second, the same naïve library was sequentially screened for phage exhibiting TM binding, internalization and then transit. Results revealed a novel set of peptides that transit across the TM to the ME in a time and temperature dependent manner. The peptides with highest transport capacities shared sequence similarities. Historically, the TM was viewed as an impermeable barrier. However, our studies reveal that it is possible to translocate peptide-linked small particles across the TM. This is the first comprehensive biopanning for the isolation of TM transiting peptidic ligands. The identified mechanism offers a new drug delivery platform into the ME." @default.
• W2296756579 created "2016-06-24" @default.
• W2296756579 creator A5001537127 @default.
• W2296756579 creator A5013796910 @default.
• W2296756579 creator A5026671565 @default.
• W2296756579 creator A5037947412 @default.
• W2296756579 creator A5085025538 @default.
• W2296756579 date "2016-03-07" @default.
• W2296756579 modified "2023-10-16" @default.
• W2296756579 title "Discovery of a Biological Mechanism of Active Transport through the Tympanic Membrane to the Middle Ear" @default.
• W2296756579 cites W111402081 @default.
• W2296756579 cites W1913862483 @default.
• W2296756579 cites W1965109237 @default.
• W2296756579 cites W1966887569 @default.
• W2296756579 cites W1967351152 @default.
• W2296756579 cites W1968307008 @default.
• W2296756579 cites W1972227970 @default.
• W2296756579 cites W1977396678 @default.
• W2296756579 cites W1983444899 @default.
• W2296756579 cites W1993721729 @default.
• W2296756579 cites W1994578277 @default.
• W2296756579 cites W1995100859 @default.
• W2296756579 cites W1997575888 @default.
• W2296756579 cites W1998162097 @default.
• W2296756579 cites W2000141520 @default.
• W2296756579 cites W2005275370 @default.
• W2296756579 cites W2006101690 @default.
• W2296756579 cites W2021166433 @default.
• W2296756579 cites W2027265512 @default.
• W2296756579 cites W2030231027 @default.
• W2296756579 cites W2030476352 @default.
• W2296756579 cites W2033167476 @default.
• W2296756579 cites W2035040170 @default.
• W2296756579 cites W2035755942 @default.
• W2296756579 cites W2045827741 @default.
• W2296756579 cites W2047405247 @default.
• W2296756579 cites W2048500179 @default.
• W2296756579 cites W2056232124 @default.
• W2296756579 cites W2064189557 @default.
• W2296756579 cites W2065303540 @default.
• W2296756579 cites W2070409252 @default.
• W2296756579 cites W2072788367 @default.
• W2296756579 cites W2090966844 @default.
• W2296756579 cites W2092327932 @default.
• W2296756579 cites W2096065225 @default.
• W2296756579 cites W2097733637 @default.
• W2296756579 cites W2098252665 @default.
• W2296756579 cites W2101271566 @default.
• W2296756579 cites W2110148332 @default.
• W2296756579 cites W2121269352 @default.
• W2296756579 cites W2148749933 @default.
• W2296756579 cites W2150500774 @default.
• W2296756579 cites W2158133029 @default.
• W2296756579 cites W2161162374 @default.
• W2296756579 cites W2166827369 @default.
• W2296756579 cites W2167091517 @default.
• W2296756579 cites W2325778454 @default.
• W2296756579 cites W2605667004 @default.
• W2296756579 cites W4211180241 @default.
• W2296756579 cites W4254695842 @default.
• W2296756579 cites W62276193 @default.
• W2296756579 doi "https://doi.org/10.1038/srep22663" @default.
• W2296756579 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4780071" @default.
• W2296756579 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/26946957" @default.
• W2296756579 hasPublicationYear "2016" @default.
• W2296756579 type Work @default.
• W2296756579 sameAs 2296756579 @default.
• W2296756579 citedByCount "21" @default.
• W2296756579 countsByYear W22967565792017 @default.
• W2296756579 countsByYear W22967565792018 @default.
• W2296756579 countsByYear W22967565792019 @default.
• W2296756579 countsByYear W22967565792020 @default.
• W2296756579 countsByYear W22967565792021 @default.
• W2296756579 countsByYear W22967565792022 @default.
• W2296756579 countsByYear W22967565792023 @default.
• W2296756579 crossrefType "journal-article" @default.
• W2296756579 hasAuthorship W2296756579A5001537127 @default.
• W2296756579 hasAuthorship W2296756579A5013796910 @default.
• W2296756579 hasAuthorship W2296756579A5026671565 @default.
• W2296756579 hasAuthorship W2296756579A5037947412 @default.
• W2296756579 hasAuthorship W2296756579A5085025538 @default.
• W2296756579 hasBestOaLocation W22967565791 @default.
• W2296756579 hasConcept C104317684 @default.
• W2296756579 hasConcept C104428545 @default.
• W2296756579 hasConcept C105702510 @default.
• W2296756579 hasConcept C12554922 @default.
• W2296756579 hasConcept C167625842 @default.
• W2296756579 hasConcept C185592680 @default.
• W2296756579 hasConcept C186268636 @default.
• W2296756579 hasConcept C2776439223 @default.
• W2296756579 hasConcept C2776471321 @default.
• W2296756579 hasConcept C2777846895 @default.
• W2296756579 hasConcept C2779281246 @default.
• W2296756579 hasConcept C501593827 @default.
• W2296756579 hasConcept C54355233 @default.
• W2296756579 hasConcept C55493867 @default.
• W2296756579 hasConcept C70721500 @default.
• W2296756579 hasConcept C86803240 @default.
• W2296756579 hasConceptScore W2296756579C104317684 @default.
• W2296756579 hasConceptScore W2296756579C104428545 @default.

• next