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- W2296776132 abstract "The UV-spectrophotometric method of analysis was proposed for simultaneous determination of sulfamethoxazole and trimethoprim. Considering the strong spectral overlap among UV-vis spectra of these compounds, a previous separation should be carried out in order to determine them by conventional spectropho- tometric techniques. Here, full-spectrum multivariate calibrations PLS and PCR methods are developed. The experimental calibration matrix was constructed with 26 samples. The concentration ranges considered were 2-15µgml-1 sulfamethoxazole and 0.4-6µgml-1 trimethoprim.Absorbance data of the calibration standards were taken between 200-400nm with UV-vis spectrophotometer. For achieving the best model, related param- eters of the model were evaluated. The optimum number of factors was selected by using the cross-validation method. The relative errors in each step were calculated. The PLS and PCR calibrations based on the raw data matrix were utilized to illustrate successfully the application of the proposed methods for analysis of the drugs in synthetic mixtures and pharmaceutical tablet. The errors obtained in the PLS method were slightly better. The errors were less than 0.1% for sulfamethoxazole and 0.9% for trimethoprim in PLS method, respectively." @default.
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- W2296776132 date "2010-01-01" @default.
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- W2296776132 title "SIMULTANEOUS SPECTROPHOTOMETRIC DETERMINATION OF SULFAMETHOXAZOLE AND TRIMETHOPRIM IN PHARMACEUTICAL PREPARATIONS BY USING MULTIVARIATE CALIBRATION METHODS" @default.
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