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- W2296787637 abstract "Diethylstilbestrol (DES), a synthetic analog of estrogen, acts as a mitotic/meiotic inhibitor via the disruption of microtubules and severely affects gametogenesis. Treatment of cells with DES results in the production of aneuploid cells and gametes. To quantify the concentration of DES in tissues, cells or subcellular fractions, an anti-DES antibody was produced conjugated to keyhole limpet hemocyanin (KLH) and a modified enzyme-linked immunosorbent assay for DES was developed. The sensitivity of the assay was 10 ng/ml of DES which is in the same range as the RIA technique, but has the advantage of not using radioactive particles in the analysis. The high correlation coefficient (r2=0.99) indicated a linear relationship between the concentration of DES in the fluid or tissue and the anti-DES antibody." @default.
- W2296787637 created "2016-06-24" @default.
- W2296787637 creator A5062174594 @default.
- W2296787637 date "1990-12-26" @default.
- W2296787637 modified "2023-09-25" @default.
- W2296787637 title "Biological Response and Quantitation of Diethylstilbestrol via Enzyme-Linked Immunosorbent Assay" @default.
- W2296787637 doi "https://doi.org/10.1021/bk-1990-0451.ch025" @default.
- W2296787637 hasPublicationYear "1990" @default.
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