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- W2296903171 abstract "Abstract A plastic dish with a wavelength-scale periodic structure at a bottom panel was integrally molded and coated with thin metal films. The integrally molded dish called plasmonic dish was applied to bioimaging under a fluorescence microscope. On the plasmonic substrate, the enhanced electric field based on a grating-coupled surface plasmon resonance (GC-SPR) can provide an enhanced fluorescence. In this study, two kinds of cells, human embryonic kidney (HEK) cells and neuronal cells, were observed in our plasmonic dish. Fluorescence images of HEK cells were above 10 times brighter than those obtained on a conventional glass-bottomed dish. Neuronal cells were successfully cultured for 10 d on the plasmonic dish integrally molded, and in fluorescence images with transmitted light, a higher contrast was obtained than in epifluorescence images. The plasmonic dish integrally molded, as well as that fabricated by the UV nanoimprint method, was also found to be useful for sensitive bioimaging." @default.
- W2296903171 created "2016-06-24" @default.
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- W2296903171 date "2016-02-22" @default.
- W2296903171 modified "2023-10-18" @default.
- W2296903171 title "Fluorescence microscopy imaging of cells with a plasmonic dish integrally molded" @default.
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- W2296903171 doi "https://doi.org/10.7567/jjap.55.03df12" @default.
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