FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2296921559> ?p ?o ?g. }

• W2296921559 abstract "This work reports an in vivo approach for identifying the function of biomineralization-related proteins. Synthetic sequences of n16N, OC-17 and perlucin with signal peptides are produced in a novel Gateway expression system for Dictyostelium under the control of the [ecmB] promoter. A fast and easy scanning electron microscopic screening method was used to differentiate on the colony level between interplay effects of the proteins expressed in the extracellular matrix (ECM). Transformed Dictyostelium, which migrated as multicellular colonies on calcite crystals and left their ECM remnants on the surface were investigated also by energy-dispersive X-ray spectroscopy (EDX). Calcium minerals with and without phosphorous accumulated very frequently within the matrix of the Dictyostelium colonies when grown on calcite. Magnesium containing phosphorous granules were observed when colonies were exposed on silica. The absence of calcium EDX signals in these cases suggests that the external calcite crystals but not living cells represent the major source of calcium in the ECM. Several features of the system provide first evidence that each protein influences the properties of the matrix in a characteristic mode. Colonies transformed with perlucin produced a matrix with cracks on the length scale of a few microns throughout the matrix patch. For colonies with OC-17, almost no cracks were observed, regardless of the length scale. The non-transformed Dictyostelium (Ax3-Orf+) produced larger cracks. The strategy presented here develops the first step toward an efficient eukaryotic screening system for the combinatorial functionalization of materials by bioengineering in close analogy to natural biomineralization concepts." @default.
• W2296921559 created "2016-06-24" @default.
• W2296921559 creator A5005380598 @default.
• W2296921559 creator A5016224157 @default.
• W2296921559 creator A5022648696 @default.
• W2296921559 creator A5025552969 @default.
• W2296921559 creator A5084730503 @default.
• W2296921559 date "2016-11-01" @default.
• W2296921559 modified "2023-09-27" @default.
• W2296921559 title "In vivo modified organic matrix for testing biomineralization-related protein functions in differentiated Dictyostelium on calcite" @default.
• W2296921559 cites W1563231805 @default.
• W2296921559 cites W1592810603 @default.
• W2296921559 cites W1596164007 @default.
• W2296921559 cites W1626599458 @default.
• W2296921559 cites W1631270093 @default.
• W2296921559 cites W1860918084 @default.
• W2296921559 cites W1879374409 @default.
• W2296921559 cites W1880245975 @default.
• W2296921559 cites W1968420547 @default.
• W2296921559 cites W1968990771 @default.
• W2296921559 cites W1970831027 @default.
• W2296921559 cites W1976251787 @default.
• W2296921559 cites W1984314191 @default.
• W2296921559 cites W1984731533 @default.
• W2296921559 cites W1986162075 @default.
• W2296921559 cites W1993423963 @default.
• W2296921559 cites W1996350907 @default.
• W2296921559 cites W1999629320 @default.
• W2296921559 cites W2000600482 @default.
• W2296921559 cites W2002487068 @default.
• W2296921559 cites W2003566398 @default.
• W2296921559 cites W2008997860 @default.
• W2296921559 cites W2009091242 @default.
• W2296921559 cites W2010809008 @default.
• W2296921559 cites W2012077697 @default.
• W2296921559 cites W2023546705 @default.
• W2296921559 cites W2024654045 @default.
• W2296921559 cites W2024967076 @default.
• W2296921559 cites W2026419188 @default.
• W2296921559 cites W2026850820 @default.
• W2296921559 cites W2031846886 @default.
• W2296921559 cites W2034341688 @default.
• W2296921559 cites W2034720620 @default.
• W2296921559 cites W2035384099 @default.
• W2296921559 cites W2037951645 @default.
• W2296921559 cites W2039908784 @default.
• W2296921559 cites W2039942900 @default.
• W2296921559 cites W2040438092 @default.
• W2296921559 cites W2041090790 @default.
• W2296921559 cites W2043111239 @default.
• W2296921559 cites W2055371745 @default.
• W2296921559 cites W2059383371 @default.
• W2296921559 cites W2059743959 @default.
• W2296921559 cites W2060135702 @default.
• W2296921559 cites W2073308003 @default.
• W2296921559 cites W2074308942 @default.
• W2296921559 cites W2082742326 @default.
• W2296921559 cites W2089063323 @default.
• W2296921559 cites W2112370094 @default.
• W2296921559 cites W2123011869 @default.
• W2296921559 cites W2127068865 @default.
• W2296921559 cites W2127392957 @default.
• W2296921559 cites W2127816425 @default.
• W2296921559 cites W2132370546 @default.
• W2296921559 cites W2133243155 @default.
• W2296921559 cites W2135528772 @default.
• W2296921559 cites W2138437559 @default.
• W2296921559 cites W2141426301 @default.
• W2296921559 cites W2141921174 @default.
• W2296921559 cites W2154977216 @default.
• W2296921559 cites W2155818894 @default.
• W2296921559 cites W2163087551 @default.
• W2296921559 cites W2315729518 @default.
• W2296921559 cites W2326638350 @default.
• W2296921559 cites W2337613320 @default.
• W2296921559 cites W2413993348 @default.
• W2296921559 cites W2493236407 @default.
• W2296921559 cites W2797540009 @default.
• W2296921559 cites W2798259643 @default.
• W2296921559 cites W3215129014 @default.
• W2296921559 cites W81325240 @default.
• W2296921559 cites W2493705158 @default.
• W2296921559 doi "https://doi.org/10.1016/j.jsb.2016.03.015" @default.
• W2296921559 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/26993464" @default.
• W2296921559 hasPublicationYear "2016" @default.
• W2296921559 type Work @default.
• W2296921559 sameAs 2296921559 @default.
• W2296921559 citedByCount "2" @default.
• W2296921559 countsByYear W22969215592016 @default.
• W2296921559 crossrefType "journal-article" @default.
• W2296921559 hasAuthorship W2296921559A5005380598 @default.
• W2296921559 hasAuthorship W2296921559A5016224157 @default.
• W2296921559 hasAuthorship W2296921559A5022648696 @default.
• W2296921559 hasAuthorship W2296921559A5025552969 @default.
• W2296921559 hasAuthorship W2296921559A5084730503 @default.
• W2296921559 hasConcept C104317684 @default.
• W2296921559 hasConcept C106487976 @default.
• W2296921559 hasConcept C12554922 @default.
• W2296921559 hasConcept C151730666 @default.
• W2296921559 hasConcept C178790620 @default.

• next