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• W2296930995 abstract "Protein assemblies are an emerging tool that is finding many biological and bioengineering applications. We here propose a method for the site-specific assembly of proteins on a twigged streptavidin (SA) polymer using streptavidin as a functional scaffold. SA was genetically appended with a G tag (sortase A recognition sequence) and a Y tag (HRP recognition sequence) on its N- and C-termini, respectively, to provide G-SA-Y. G-SA-Y was polymerized using HPR-mediated tyrosine coupling, then fluorescent proteins were immobilized on the polymer by biotin-SA affinity and sortase A-mediated ligation. Fluorescence measurements showed that the proteins were immobilized in close proximity to each other. Hydrolyzing enzymes were also functionally assembled on the G-SA-Y polymer. The site-specific assembly of proteins on twigged SA polymer may find new applications in various biological and bioengineering fields." @default.
• W2296930995 created "2016-06-24" @default.
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• W2296930995 date "2016-05-01" @default.
• W2296930995 modified "2023-10-18" @default.
• W2296930995 title "Twigged streptavidin polymer as a scaffold for protein assembly" @default.
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• W2296930995 doi "https://doi.org/10.1016/j.jbiotec.2016.03.030" @default.
• W2296930995 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/27002233" @default.
• W2296930995 hasPublicationYear "2016" @default.
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