Matches in SemOpenAlex for { <https://semopenalex.org/work/W2296938981> ?p ?o ?g. }
- W2296938981 endingPage "2053" @default.
- W2296938981 startingPage "2043" @default.
- W2296938981 abstract "Translesion synthesis (TLS) is a mutagenic branch of cellular DNA damage tolerance that enables bypass replication over DNA lesions carried out by specialized low-fidelity DNA polymerases. The replicative bypass of most types of DNA damage is performed in a two-step process of Rev1/Polζ-dependent TLS. In the first step, a Y-family TLS enzyme, typically Polη, Polι, or Polκ, inserts a nucleotide across a DNA lesion. In the second step, a four-subunit B-family DNA polymerase Polζ (Rev3/Rev7/PolD2/PolD3 complex) extends the distorted DNA primer-template. The coordinated action of error-prone TLS enzymes is regulated through their interactions with the two scaffold proteins, the sliding clamp PCNA and the TLS polymerase Rev1. Rev1 interactions with all other TLS enzymes are mediated by its C-terminal domain (Rev1-CT), which can simultaneously bind the Rev7 subunit of Polζ and Rev1-interacting regions (RIRs) from Polη, Polι, or Polκ. In this work, we identified a previously unknown RIR motif in the C-terminal part of PolD3 subunit of Polζ whose interaction with the Rev1-CT is among the tightest mediated by RIR motifs. Three-dimensional structure of the Rev1-CT/PolD3-RIR complex determined by NMR spectroscopy revealed a structural basis for the relatively high affinity of this interaction. The unexpected discovery of PolD3-RIR motif suggests a mechanism of “inserter” to “extender” DNA polymerase switch upon Rev1/Polζ-dependent TLS, in which the PolD3-RIR binding to the Rev1-CT (i) helps displace the “inserter” Polη, Polι, or Polκ from its complex with Rev1, and (ii) facilitates assembly of the four-subunit “extender” Polζ through simultaneous interaction of Rev1-CT with Rev7 and PolD3 subunits." @default.
- W2296938981 created "2016-06-24" @default.
- W2296938981 creator A5008065534 @default.
- W2296938981 creator A5017863285 @default.
- W2296938981 creator A5027003258 @default.
- W2296938981 creator A5035401129 @default.
- W2296938981 creator A5081995817 @default.
- W2296938981 creator A5083844605 @default.
- W2296938981 creator A5090953868 @default.
- W2296938981 date "2016-03-24" @default.
- W2296938981 modified "2023-09-24" @default.
- W2296938981 title "Interaction between the Rev1 C-Terminal Domain and the PolD3 Subunit of Polζ Suggests a Mechanism of Polymerase Exchange upon Rev1/Polζ-Dependent Translesion Synthesis" @default.
- W2296938981 cites W1621604326 @default.
- W2296938981 cites W1772122236 @default.
- W2296938981 cites W1840855988 @default.
- W2296938981 cites W1904872562 @default.
- W2296938981 cites W1963667773 @default.
- W2296938981 cites W1968437581 @default.
- W2296938981 cites W1980578402 @default.
- W2296938981 cites W1980745500 @default.
- W2296938981 cites W1982406824 @default.
- W2296938981 cites W1982453626 @default.
- W2296938981 cites W1984816647 @default.
- W2296938981 cites W1985133799 @default.
- W2296938981 cites W1991646855 @default.
- W2296938981 cites W1995017064 @default.
- W2296938981 cites W1996368299 @default.
- W2296938981 cites W1996443829 @default.
- W2296938981 cites W1997029264 @default.
- W2296938981 cites W1997262127 @default.
- W2296938981 cites W1997803780 @default.
- W2296938981 cites W1998604023 @default.
- W2296938981 cites W2001209925 @default.
- W2296938981 cites W2002503761 @default.
- W2296938981 cites W2005129633 @default.
- W2296938981 cites W2006470414 @default.
- W2296938981 cites W2011647977 @default.
- W2296938981 cites W2018179063 @default.
- W2296938981 cites W2020136215 @default.
- W2296938981 cites W2020866179 @default.
- W2296938981 cites W2024388814 @default.
- W2296938981 cites W2024703739 @default.
- W2296938981 cites W2029422499 @default.
- W2296938981 cites W2032124996 @default.
- W2296938981 cites W2035521675 @default.
- W2296938981 cites W2040860750 @default.
- W2296938981 cites W2043418439 @default.
- W2296938981 cites W2051857414 @default.
- W2296938981 cites W2053202752 @default.
- W2296938981 cites W2055869110 @default.
- W2296938981 cites W2057218806 @default.
- W2296938981 cites W2061990742 @default.
- W2296938981 cites W2062487397 @default.
- W2296938981 cites W2062739067 @default.
- W2296938981 cites W2063240589 @default.
- W2296938981 cites W2064328955 @default.
- W2296938981 cites W2064614219 @default.
- W2296938981 cites W2065626377 @default.
- W2296938981 cites W2066526435 @default.
- W2296938981 cites W2072747624 @default.
- W2296938981 cites W2072969527 @default.
- W2296938981 cites W2073745636 @default.
- W2296938981 cites W2085585779 @default.
- W2296938981 cites W2088486043 @default.
- W2296938981 cites W2092869952 @default.
- W2296938981 cites W2094549019 @default.
- W2296938981 cites W2096625253 @default.
- W2296938981 cites W2096640675 @default.
- W2296938981 cites W2098418459 @default.
- W2296938981 cites W2099367992 @default.
- W2296938981 cites W2101101375 @default.
- W2296938981 cites W2101364159 @default.
- W2296938981 cites W2102091313 @default.
- W2296938981 cites W2109484769 @default.
- W2296938981 cites W2111616806 @default.
- W2296938981 cites W2112418305 @default.
- W2296938981 cites W2119301405 @default.
- W2296938981 cites W2120269671 @default.
- W2296938981 cites W2126493139 @default.
- W2296938981 cites W2130892534 @default.
- W2296938981 cites W2133071480 @default.
- W2296938981 cites W2135858046 @default.
- W2296938981 cites W2136341398 @default.
- W2296938981 cites W2138293368 @default.
- W2296938981 cites W2139137226 @default.
- W2296938981 cites W2139168208 @default.
- W2296938981 cites W2140230014 @default.
- W2296938981 cites W2142881120 @default.
- W2296938981 cites W2148509268 @default.
- W2296938981 cites W2156435713 @default.
- W2296938981 cites W2167668429 @default.
- W2296938981 cites W2169821755 @default.
- W2296938981 cites W2169950973 @default.
- W2296938981 cites W2224030007 @default.
- W2296938981 cites W2315214870 @default.
- W2296938981 cites W4294326239 @default.
- W2296938981 doi "https://doi.org/10.1021/acs.biochem.5b01282" @default.
- W2296938981 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4898654" @default.