Matches in SemOpenAlex for { <https://semopenalex.org/work/W2296969764> ?p ?o ?g. }
- W2296969764 abstract "Abstract Building a sophisticated protein nano-assembly requires a method for linking protein components in a predictable and stable structure. Most of the cross linkers available have flexible spacers. Because of this, the linked hybrids have significant structural flexibility and the relative structure between their two components is largely unpredictable. Here we describe a method of connecting two proteins via a ‘fusion α helix’ formed by joining two pre-existing helices into a single extended helix. Because simple ligation of two helices does not guarantee the formation of a continuous helix, we used EY-CBS, a synthetic cross linker that has been shown to react selectively with cysteines in α-helices, to stabilize the connecting helix. Formation and stabilization of the fusion helix was confirmed by determining the crystal structures of the fusion proteins with and without bound EY-CBS. Our method should be widely applicable for linking protein building blocks to generate predictable structures." @default.
- W2296969764 created "2016-06-24" @default.
- W2296969764 creator A5017735053 @default.
- W2296969764 creator A5032797824 @default.
- W2296969764 creator A5040482204 @default.
- W2296969764 creator A5043986899 @default.
- W2296969764 creator A5059691183 @default.
- W2296969764 creator A5070548246 @default.
- W2296969764 creator A5078226105 @default.
- W2296969764 creator A5087762681 @default.
- W2296969764 date "2016-03-16" @default.
- W2296969764 modified "2023-10-02" @default.
- W2296969764 title "Connecting two proteins using a fusion alpha helix stabilized by a chemical cross linker" @default.
- W2296969764 cites W1578789128 @default.
- W2296969764 cites W1946074662 @default.
- W2296969764 cites W1975898100 @default.
- W2296969764 cites W1979572873 @default.
- W2296969764 cites W1981227353 @default.
- W2296969764 cites W1987159249 @default.
- W2296969764 cites W1987515055 @default.
- W2296969764 cites W1989346024 @default.
- W2296969764 cites W2004130939 @default.
- W2296969764 cites W2004797045 @default.
- W2296969764 cites W2005174659 @default.
- W2296969764 cites W2008142453 @default.
- W2296969764 cites W2008728387 @default.
- W2296969764 cites W2010753961 @default.
- W2296969764 cites W2015526636 @default.
- W2296969764 cites W2023697479 @default.
- W2296969764 cites W2039399096 @default.
- W2296969764 cites W2045511259 @default.
- W2296969764 cites W2051546721 @default.
- W2296969764 cites W2051845341 @default.
- W2296969764 cites W2057091413 @default.
- W2296969764 cites W2061095840 @default.
- W2296969764 cites W2062801269 @default.
- W2296969764 cites W2063512060 @default.
- W2296969764 cites W2063755536 @default.
- W2296969764 cites W2074394454 @default.
- W2296969764 cites W2074464198 @default.
- W2296969764 cites W2075987376 @default.
- W2296969764 cites W2080231167 @default.
- W2296969764 cites W2084214674 @default.
- W2296969764 cites W2084938664 @default.
- W2296969764 cites W2086456499 @default.
- W2296969764 cites W2089046899 @default.
- W2296969764 cites W2090643737 @default.
- W2296969764 cites W2104477830 @default.
- W2296969764 cites W2106937142 @default.
- W2296969764 cites W2113495611 @default.
- W2296969764 cites W2116477640 @default.
- W2296969764 cites W2124026197 @default.
- W2296969764 cites W2124611811 @default.
- W2296969764 cites W2163341755 @default.
- W2296969764 cites W2165238581 @default.
- W2296969764 cites W2180229411 @default.
- W2296969764 cites W349268771 @default.
- W2296969764 doi "https://doi.org/10.1038/ncomms11031" @default.
- W2296969764 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4799363" @default.
- W2296969764 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/26980593" @default.
- W2296969764 hasPublicationYear "2016" @default.
- W2296969764 type Work @default.
- W2296969764 sameAs 2296969764 @default.
- W2296969764 citedByCount "18" @default.
- W2296969764 countsByYear W22969697642016 @default.
- W2296969764 countsByYear W22969697642017 @default.
- W2296969764 countsByYear W22969697642018 @default.
- W2296969764 countsByYear W22969697642019 @default.
- W2296969764 countsByYear W22969697642020 @default.
- W2296969764 countsByYear W22969697642022 @default.
- W2296969764 countsByYear W22969697642023 @default.
- W2296969764 crossrefType "journal-article" @default.
- W2296969764 hasAuthorship W2296969764A5017735053 @default.
- W2296969764 hasAuthorship W2296969764A5032797824 @default.
- W2296969764 hasAuthorship W2296969764A5040482204 @default.
- W2296969764 hasAuthorship W2296969764A5043986899 @default.
- W2296969764 hasAuthorship W2296969764A5059691183 @default.
- W2296969764 hasAuthorship W2296969764A5070548246 @default.
- W2296969764 hasAuthorship W2296969764A5078226105 @default.
- W2296969764 hasAuthorship W2296969764A5087762681 @default.
- W2296969764 hasBestOaLocation W22969697641 @default.
- W2296969764 hasConcept C104317684 @default.
- W2296969764 hasConcept C105795698 @default.
- W2296969764 hasConcept C10919887 @default.
- W2296969764 hasConcept C111919701 @default.
- W2296969764 hasConcept C123894998 @default.
- W2296969764 hasConcept C12554922 @default.
- W2296969764 hasConcept C138885662 @default.
- W2296969764 hasConcept C158525013 @default.
- W2296969764 hasConcept C184538305 @default.
- W2296969764 hasConcept C185592680 @default.
- W2296969764 hasConcept C18903297 @default.
- W2296969764 hasConcept C2778530040 @default.
- W2296969764 hasConcept C2779965526 @default.
- W2296969764 hasConcept C2780557392 @default.
- W2296969764 hasConcept C2780598303 @default.
- W2296969764 hasConcept C33923547 @default.
- W2296969764 hasConcept C40767141 @default.
- W2296969764 hasConcept C41008148 @default.
- W2296969764 hasConcept C41895202 @default.