FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2297260892> ?p ?o ?g. }

• W2297260892 endingPage "1774" @default.
• W2297260892 startingPage "1774" @default.
• W2297260892 abstract "HomePlant DiseaseVol. 100, No. 8Association of 16SrII-D Phytoplasma With Phyllody Disease in Sesame (Sesamum indicum) in Telangana From Southern India PreviousNext DISEASE NOTES OPENOpen Access licenseAssociation of 16SrII-D Phytoplasma With Phyllody Disease in Sesame (Sesamum indicum) in Telangana From Southern IndiaI. Pamei and R. MakandarI. PameiSearch for more papers by this author and R. MakandarSearch for more papers by this authorAffiliationsAuthors and Affiliations I. Pamei R. Makandar , Department of Plant Sciences, School of Life Sciences, University of Hyderabad, Gachibowli, Hyderabad 500046, India. Published Online:9 May 2016https://doi.org/10.1094/PDIS-11-15-1348-PDNAboutSections ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmailWechat Sesame (Sesamum indicum L.) is an important and ancient oil-seed crop, domesticated over 3,000 years ago. Leaf-like flower (phyllody), virescence, and proliferation, which were typical symptoms of phytoplasma infection, were observed in sesame breeding lines in Hyderabad, Telangana, India during the cropping period between July and October 2012-14. The sesame plants were monitored for symptom development under natural infection in field conditions and leaf samples from three symptomatic and three asymptomatic plants were collected. Though leaf samples from asymptomatic and symptomatic plants were analyzed from 2012-14, the experimental figures presented correspond to the samples collected in September 2014. Total genomic DNA was isolated from the samples following a CTAB DNA extraction method (Smart et al. 1996). Genomic PCR with phytoplasma-specific P1/Tint primer pair (Smart et al. 1996) followed by nested PCR primer R16F2n/R16R2 (Lee et al. 1998) resulted in a PCR product of 1.6 and 1.25 kb, respectively, for all the three symptomatic samples whereas amplification of 16S rRNA gene was not detected in asymptomatic samples used as negative controls. The 1.25 kb DNA fragment obtained from three symptomatic samples was purified using the Nucleospin Gel and PCR Clean-up kit (Macherey Nagel), cloned into pGEMT Easy vector I (Promega) as per manufacturer’s instructions, and sequenced using M13 universal primer on an ABI 3730Xl automated sequencer at SciGenom sequencing services (SciGenom Labs, Cochin, India). The DNA sequences obtained from these samples share >99% similarity with each other and one of these sequences is deposited in GenBank with accession number KP297862. NCBI BLASTn analysis of KP297862 revealed 100% identity with Echinacea witches’-broom phytoplasma strain EWB6 (JF340080), papaya yellow crinkle disease phytoplasma (Y10097), and sesame phyllody phytoplasma strain Delhi (KC920750). Though phylogenetic analysis of KP297862 with representative sequences retrieved from GenBank revealed close proximity to Candidatus Phytoplasma aurantifolia (U15442), which belongs to 16SrII group (unpublished data); however, using iPhyClassifier (Zhao et al. 2009) for subgroup identification revealed sequence relatedness with similarity coefficient of 1.00 to Ca. P. australasia (Y10097), which represents 16SrII-D. An in silico RFLP digestion using 17 restriction enzymes followed by a real-RFLP digestion using six key restriction enzymes, viz., AluI, BamHI, EcoRI, KpnI, RsaI, and TaqI confirmed the phytoplasma identified in our study as 16SrII-D subgroup. Though phytoplasma association with sesame was identified in different regions of the country, like the subgroups 16SrI-B and 16SrII-C from Uttar Pradesh, 16SrI-B subgroup from Delhi and Bihar in northern India (Nabi et al. 2015), and subgroup 16SrI in Karnataka (Manjunatha et al. 2012) from southern India, the phytoplasma identified in our study belongs to 16SrII-D subgroup, which is being reported for the first time in sesame from Telangana in India. Early infection of sesame plants leads to total loss of seed production. Detection of the phytoplasma during early stages of plant growth would help in reducing the cultivation costs and for effective crop breeding program.References:Lee, I. M., et al. 1998. Int. J. Syst. Bacteriol. 48:1153. https://doi.org/10.1099/00207713-48-4-1153 Crossref, Google ScholarManjunatha, N., et al. 2012. Phytopath. Moll. 2:29. Crossref, Google ScholarNabi, S. U., et al. 2015. Indian Phytopath. 68:112. Google ScholarSmart, C. D., et al. 1996. Appl. Environ. Microbiol. 62:2988. Crossref, ISI, Google ScholarZhao, Y., et al. 2009. Int. J. Syst. Evol. Microbiol. 59:2582. https://doi.org/10.1099/ijs.0.010249-0 Crossref, ISI, Google ScholarDetailsFiguresLiterature CitedRelated Vol. 100, No. 8 August 2016SubscribeISSN:0191-2917e-ISSN:1943-7692 Metrics Article History Issue Date: 22 Jul 2016Published: 9 May 2016First Look: 15 Mar 2016Accepted: 27 Feb 2016 Pages: 1774-1774 Information© 2016 The American Phytopathological SocietyCited byComparative proteome analysis reveals the role of negative floral regulators and defense-related genes in phytoplasma infected sesame21 February 2022 | Protoplasma, Vol. 259, No. 6Comparative Transcriptome Provides a New Insight into Floral Regulation and Defense Response Against Phytoplasma in Sesame (Sesamum indicum L.)22 January 2022 | Plant Molecular Biology Reporter, Vol. 40, No. 3Molecular detection and characterization of a stolbur phytoplasma associated with Narcissus tazetta phyllody in Iran27 February 2018 | Journal of Phytopathology, Vol. 166, No. 5Association of two groups of phytoplasma with various symptoms in some wooden and herbaceous plants29 January 2018 | Journal of Phytopathology, Vol. 166, No. 4" @default.
• W2297260892 created "2016-06-24" @default.
• W2297260892 creator A5023985399 @default.
• W2297260892 creator A5073692638 @default.
• W2297260892 date "2016-08-01" @default.
• W2297260892 modified "2023-10-14" @default.
• W2297260892 title "Association of 16SrII-D Phytoplasma With Phyllody Disease in Sesame (<i>Sesamum indicum</i>) in Telangana From Southern India" @default.
• W2297260892 cites W2064108992 @default.
• W2297260892 cites W2077242675 @default.
• W2297260892 cites W2145409763 @default.
• W2297260892 cites W2156541321 @default.
• W2297260892 doi "https://doi.org/10.1094/pdis-11-15-1348-pdn" @default.
• W2297260892 hasPublicationYear "2016" @default.
• W2297260892 type Work @default.
• W2297260892 sameAs 2297260892 @default.
• W2297260892 citedByCount "6" @default.
• W2297260892 countsByYear W22972608922018 @default.
• W2297260892 countsByYear W22972608922020 @default.
• W2297260892 countsByYear W22972608922022 @default.
• W2297260892 countsByYear W22972608922023 @default.
• W2297260892 crossrefType "journal-article" @default.
• W2297260892 hasAuthorship W2297260892A5023985399 @default.
• W2297260892 hasAuthorship W2297260892A5073692638 @default.
• W2297260892 hasBestOaLocation W22972608921 @default.
• W2297260892 hasConcept C104317684 @default.
• W2297260892 hasConcept C137580998 @default.
• W2297260892 hasConcept C144027150 @default.
• W2297260892 hasConcept C168007829 @default.
• W2297260892 hasConcept C2776331994 @default.
• W2297260892 hasConcept C2778533604 @default.
• W2297260892 hasConcept C2780760702 @default.
• W2297260892 hasConcept C2780928516 @default.
• W2297260892 hasConcept C42972112 @default.
• W2297260892 hasConcept C49105822 @default.
• W2297260892 hasConcept C55493867 @default.
• W2297260892 hasConcept C59822182 @default.
• W2297260892 hasConcept C6557445 @default.
• W2297260892 hasConcept C71924100 @default.
• W2297260892 hasConcept C86803240 @default.
• W2297260892 hasConceptScore W2297260892C104317684 @default.
• W2297260892 hasConceptScore W2297260892C137580998 @default.
• W2297260892 hasConceptScore W2297260892C144027150 @default.
• W2297260892 hasConceptScore W2297260892C168007829 @default.
• W2297260892 hasConceptScore W2297260892C2776331994 @default.
• W2297260892 hasConceptScore W2297260892C2778533604 @default.
• W2297260892 hasConceptScore W2297260892C2780760702 @default.
• W2297260892 hasConceptScore W2297260892C2780928516 @default.
• W2297260892 hasConceptScore W2297260892C42972112 @default.
• W2297260892 hasConceptScore W2297260892C49105822 @default.
• W2297260892 hasConceptScore W2297260892C55493867 @default.
• W2297260892 hasConceptScore W2297260892C59822182 @default.
• W2297260892 hasConceptScore W2297260892C6557445 @default.
• W2297260892 hasConceptScore W2297260892C71924100 @default.
• W2297260892 hasConceptScore W2297260892C86803240 @default.
• W2297260892 hasIssue "8" @default.
• W2297260892 hasLocation W22972608921 @default.
• W2297260892 hasOpenAccess W2297260892 @default.
• W2297260892 hasPrimaryLocation W22972608921 @default.
• W2297260892 hasRelatedWork W1998047689 @default.
• W2297260892 hasRelatedWork W2070529246 @default.
• W2297260892 hasRelatedWork W2077242675 @default.
• W2297260892 hasRelatedWork W2110098179 @default.
• W2297260892 hasRelatedWork W2167509731 @default.
• W2297260892 hasRelatedWork W2896514392 @default.
• W2297260892 hasRelatedWork W2968645470 @default.
• W2297260892 hasRelatedWork W3193995875 @default.
• W2297260892 hasRelatedWork W4385802547 @default.
• W2297260892 hasRelatedWork W8073527 @default.
• W2297260892 hasVolume "100" @default.
• W2297260892 isParatext "false" @default.
• W2297260892 isRetracted "false" @default.
• W2297260892 magId "2297260892" @default.
• W2297260892 workType "article" @default.