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- W2297573635 abstract "With the advancement in microfluidics, the overall cost of polymerase chain reaction (PCR) test can be reduced significantly by using simpler device and minimal volume of samples and reagents. In this paper, a novel rotary-linear motion PCR device was designed, fabricated and tested for various contagious tropical diseases. The design incorporates the advantages of stationary chamber, continuous flow and also the oscillating flow designs. Instead of flowing samples to different static temperature zones using an external pump, this innovative design simply moves the samples in a chamber by using a novel rotary linear motion system. The results show that PCR amplification can be performed in less than 30 minutes compared to 90 minutes using a conventional device. For this, only 5μl of samples is required to perform the PCR compared to 20 μl using conventional device. The device is also able to perform PCRs on multiple samples with three different annealing temperatures in a single experiment." @default.
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- W2297573635 date "2010-01-01" @default.
- W2297573635 modified "2023-09-23" @default.
- W2297573635 title "PCRDisc: Low Cost Molecular Diagnostics Using Microfluidics Platform" @default.
- W2297573635 hasPublicationYear "2010" @default.
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