FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2297786012> ?p ?o ?g. }

• W2297786012 endingPage "e0150727" @default.
• W2297786012 startingPage "e0150727" @default.
• W2297786012 abstract "Mesoporous bioactive nanoparticles (MBNs) have been developed as promising additives to various types of bone or dentin regenerative material. However, biofunctionality of MBNs as dentin regenerative additive to dental materials have rarely been studied. We investigated the uptake efficiency of MBNs-NH2 with their endocytosis pathway and the role of MBNs-NH2 in odontogenic differentiation to clarify inherent biofunctionality. MBNs were fabricated by sol-gel synthesis, and 3% APTES was used to aminate these nanoparticles (MBNs-NH2) to reverse their charge from negative to positive. To characterize the MBNs-NH2, TEM, XRD, FTIR, zeta(ξ)-potential measurements, and Brunauer-Emmett-Teller analysis were performed. After primary cultured rat dental pulp stem cells (rDPSCs) were incubated with various concentrations of MBNs-NH2, stem cell viability (24 hours) with or without differentiated media, internalization of MBNs-NH2 in rDPSCs (~4 hours) via specific endocytosis pathway, intra or extracellular ion concentration and odontoblastic differentiation (~28 days) were investigated. Incubation with up to 50 μg/mL of MBNs-NH2 had no effect on rDPSCs viability with differentiated media (p>0.05). The internalization of MBNs-NH2 in rDPSCs was determined about 92% after 4 hours of incubation. Uptake was significantly decreased with ATP depletion and after 1 hour of pre-treatment with the inhibitor of macropinocytosis (p<0.05). There was significant increase of intracellular Ca and Si ion concentration in MBNs-NH2 treated cells compared to no-treated counterpart (p<0.05). The expression of odontogenic-related genes (BSP, COL1A, DMP-1, DSPP, and OCN) and the capacity for biomineralization (based on alkaline phosphatase activity and alizarin red staining) were significantly upregulated with MBNs-NH2. These results indicate that MBNs-NH2 induce odontogenic differentiation of rDPSCs and may serve as a potential dentin regenerative additive to dental material for promoting odontoblast differentiation." @default.
• W2297786012 created "2016-06-24" @default.
• W2297786012 creator A5001546265 @default.
• W2297786012 creator A5014129887 @default.
• W2297786012 creator A5035000624 @default.
• W2297786012 creator A5080238951 @default.
• W2297786012 date "2016-03-14" @default.
• W2297786012 modified "2023-10-17" @default.
• W2297786012 title "Effect of Aminated Mesoporous Bioactive Glass Nanoparticles on the Differentiation of Dental Pulp Stem Cells" @default.
• W2297786012 cites W1581030122 @default.
• W2297786012 cites W1804950958 @default.
• W2297786012 cites W1881573787 @default.
• W2297786012 cites W1918183249 @default.
• W2297786012 cites W1966115180 @default.
• W2297786012 cites W1978519286 @default.
• W2297786012 cites W1981130860 @default.
• W2297786012 cites W1982507096 @default.
• W2297786012 cites W1988099089 @default.
• W2297786012 cites W1993458172 @default.
• W2297786012 cites W2002936944 @default.
• W2297786012 cites W2004318971 @default.
• W2297786012 cites W2004618997 @default.
• W2297786012 cites W2005222083 @default.
• W2297786012 cites W2009773677 @default.
• W2297786012 cites W2013262011 @default.
• W2297786012 cites W2015813529 @default.
• W2297786012 cites W2017258814 @default.
• W2297786012 cites W2017593971 @default.
• W2297786012 cites W2018084034 @default.
• W2297786012 cites W2021364061 @default.
• W2297786012 cites W2030829906 @default.
• W2297786012 cites W2031964302 @default.
• W2297786012 cites W2034602186 @default.
• W2297786012 cites W2035004116 @default.
• W2297786012 cites W2036450884 @default.
• W2297786012 cites W2036958647 @default.
• W2297786012 cites W2037180810 @default.
• W2297786012 cites W2040471979 @default.
• W2297786012 cites W2041226774 @default.
• W2297786012 cites W2042345384 @default.
• W2297786012 cites W2042602209 @default.
• W2297786012 cites W2048057810 @default.
• W2297786012 cites W2050555768 @default.
• W2297786012 cites W2058657293 @default.
• W2297786012 cites W2062820788 @default.
• W2297786012 cites W2064081011 @default.
• W2297786012 cites W2071187606 @default.
• W2297786012 cites W2078690041 @default.
• W2297786012 cites W2079793032 @default.
• W2297786012 cites W2084378924 @default.
• W2297786012 cites W2086699454 @default.
• W2297786012 cites W2086756165 @default.
• W2297786012 cites W2091104036 @default.
• W2297786012 cites W2091962478 @default.
• W2297786012 cites W2094543301 @default.
• W2297786012 cites W2099947475 @default.
• W2297786012 cites W2104828689 @default.
• W2297786012 cites W2113972541 @default.
• W2297786012 cites W2116581961 @default.
• W2297786012 cites W2118510407 @default.
• W2297786012 cites W2120949195 @default.
• W2297786012 cites W2122496291 @default.
• W2297786012 cites W2125745917 @default.
• W2297786012 cites W2128153968 @default.
• W2297786012 cites W2131388166 @default.
• W2297786012 cites W2136590358 @default.
• W2297786012 cites W2144460974 @default.
• W2297786012 cites W2145133103 @default.
• W2297786012 cites W2147070666 @default.
• W2297786012 cites W2150632230 @default.
• W2297786012 cites W2151127050 @default.
• W2297786012 cites W2152617921 @default.
• W2297786012 cites W2152752812 @default.
• W2297786012 cites W2152953685 @default.
• W2297786012 cites W2156661377 @default.
• W2297786012 cites W2157367887 @default.
• W2297786012 cites W2158766613 @default.
• W2297786012 cites W2159860995 @default.
• W2297786012 cites W2160113246 @default.
• W2297786012 cites W2166398004 @default.
• W2297786012 cites W2169678660 @default.
• W2297786012 cites W2255772379 @default.
• W2297786012 cites W2284748747 @default.
• W2297786012 cites W2319850974 @default.
• W2297786012 cites W2332801624 @default.
• W2297786012 cites W3085953120 @default.
• W2297786012 cites W83148200 @default.
• W2297786012 doi "https://doi.org/10.1371/journal.pone.0150727" @default.
• W2297786012 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4790939" @default.
• W2297786012 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/26974668" @default.
• W2297786012 hasPublicationYear "2016" @default.
• W2297786012 type Work @default.
• W2297786012 sameAs 2297786012 @default.
• W2297786012 citedByCount "55" @default.
• W2297786012 countsByYear W22977860122016 @default.
• W2297786012 countsByYear W22977860122017 @default.
• W2297786012 countsByYear W22977860122018 @default.
• W2297786012 countsByYear W22977860122019 @default.

• next