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- W2298063092 abstract "Mannanase from Bacillus sp. TM01 was purified and characterized. The crude extract was purified through two steps of liquid column chromatography; i.e. CM-Sephadex C50 ion-exchange column and Sephadex G-100 gel filtration. The molecular weight was 45 kDa. Purified TM01 mannanase performed maximal activity at pH 7 and was highly stable at a range of pH 5 to pH7. In the case of temperature, it was most active at 60oC and was highly stable at the range of 40oC to 60oC. After 2-hr storage under the conditions of pH6-pH7 or that of 40oC - 60oC, the purified TM01 mannanase could retain higher than 80% of its original activity. Higher than 70% of the original activity was obtained when the reactions were carried out in the present of inorganic ions, namely, Cu2+, Fe2+, K+, Mn2+, Mg2 and Zn2+. Storing the enzyme in the present of such ions for 1 hr prior to standard assay could cause reduction in the activity by less than 30% except for that in the presence of Zn2+ which did so by about 55%. Bacillus sp. TM01 was found to be cultivated well in chicken soup medium (CSM) containing 0.5% (w/v) of chicken soup cube and 1.0% (w/v) of soybean meal. The 24-hr culture at 37oC was found to contain 681.5 U/ml of mannanase, 202.1 U/ml of xylanase, 87.7 U/ml of cellulase, 803.9 U/ml of amylase, 81.0 mU/ml of protease and 5.5 mU/ml of phytase." @default.
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- W2298063092 date "2013-09-01" @default.
- W2298063092 modified "2023-09-25" @default.
- W2298063092 title "Purification and Characterization of Bacillus sp. TM01 Mannanase and Its Production in Chicken Soup Medium" @default.
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