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• W2298103687 endingPage "2319" @default.
• W2298103687 startingPage "2312" @default.
• W2298103687 abstract "ABSTRACT The thermophilic acetogenic bacterium Thermoanaerobacter kivui , previously described not to use carbon monoxide as a carbon and energy source, was adapted to grow on CO. This was achieved by using a preculture grown on H 2 plus CO 2 and by increasing the CO concentration in small, 10% increments. T. kivui was finally able to grow within a 100% CO atmosphere. Growth on CO was found in complex and mineral media, and vitamins were not required. Carbon monoxide consumption was accompanied by acetate and hydrogen production. Cells also grew on synthesis gas (syngas) with the simultaneous use of CO and H 2 coupled to acetate production. CO oxidation in resting cells was coupled to hydrogen and acetate production and accompanied by the synthesis of ATP. A protonophore abolished ATP synthesis but stimulated H 2 production, which is consistent with a chemiosmotic mechanism of ATP synthesis. Hydrogenase activity was highest in crude extracts of CO-grown cells, and carbon monoxide dehydrogenase (CODH) activity was highest in H 2 -plus-CO 2 - or CO-grown cells. The genome of T. kivui harbors two CODH gene clusters, and both CODH proteins were present in crude extracts, but one CODH was more prevalent in crude extracts from CO-grown cells." @default.
• W2298103687 created "2016-06-24" @default.
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• W2298103687 date "2016-04-15" @default.
• W2298103687 modified "2023-10-17" @default.
• W2298103687 title "CO Metabolism in the Thermophilic Acetogen Thermoanaerobacter kivui" @default.
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• W2298103687 doi "https://doi.org/10.1128/aem.00122-16" @default.
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• W2298103687 hasPublicationYear "2016" @default.
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