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• W2298491177 abstract "Applications of the CRISPR-Cas9 system to edit the genome have widely expanded to include DNA gene knock-out, deletions, chromosomal rearrangements, RNA editing and genome-wide screenings. Here we show the application of CRISPR-Cas9 technology to edit the mouse and human immunoglobulin (Ig) genes. By delivering Cas9 and guide-RNA (gRNA) with retro- or lenti-virus to IgM(+) mouse B cells and hybridomas, we induce class-switch recombination (CSR) of the IgH chain to the desired subclass. Similarly, we induce CSR in all human B cell lines tested with high efficiency to targeted IgH subclass. Finally, we engineer mouse hybridomas to secrete Fab' fragments instead of the whole Ig. Our results indicate that Ig genes in mouse and human cells can be edited to obtain any desired IgH switching helpful to study the biology of normal and lymphoma B cells. We also propose applications that could transform the technology of antibody production." @default.
• W2298491177 created "2016-06-24" @default.
• W2298491177 creator A5015873287 @default.
• W2298491177 creator A5066463807 @default.
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• W2298491177 date "2016-03-09" @default.
• W2298491177 modified "2023-10-17" @default.
• W2298491177 title "Editing of mouse and human immunoglobulin genes by CRISPR-Cas9 system" @default.
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• W2298491177 doi "https://doi.org/10.1038/ncomms10934" @default.
• W2298491177 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4786874" @default.
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• W2298491177 hasPublicationYear "2016" @default.
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