Matches in SemOpenAlex for { <https://semopenalex.org/work/W2298532810> ?p ?o ?g. }
- W2298532810 endingPage "e1005930" @default.
- W2298532810 startingPage "e1005930" @default.
- W2298532810 abstract "The exclusive localization of the histone H3 variant CENP-A to centromeres is essential for accurate chromosome segregation. Ubiquitin-mediated proteolysis helps to ensure that CENP-A does not mislocalize to euchromatin, which can lead to genomic instability. Consistent with this, overexpression of the budding yeast CENP-ACse4 is lethal in cells lacking Psh1, the E3 ubiquitin ligase that targets CENP-ACse4 for degradation. To identify additional mechanisms that prevent CENP-ACse4 misincorporation and lethality, we analyzed the genome-wide mislocalization pattern of overexpressed CENP-ACse4 in the presence and absence of Psh1 by chromatin immunoprecipitation followed by high throughput sequencing. We found that ectopic CENP-ACse4 is enriched at promoters that contain histone H2A.ZHtz1 nucleosomes, but that H2A.ZHtz1 is not required for CENP-ACse4 mislocalization. Instead, the INO80 complex, which removes H2A.ZHtz1 from nucleosomes, promotes the ectopic deposition of CENP-ACse4. Transcriptional profiling revealed gene expression changes in the psh1Δ cells overexpressing CENP-ACse4. The down-regulated genes are enriched for CENP-ACse4 mislocalization to promoters, while the up-regulated genes correlate with those that are also transcriptionally up-regulated in an htz1Δ strain. Together, these data show that regulating centromeric nucleosome localization is not only critical for maintaining centromere function, but also for ensuring accurate promoter function and transcriptional regulation." @default.
- W2298532810 created "2016-06-24" @default.
- W2298532810 creator A5005300159 @default.
- W2298532810 creator A5030116295 @default.
- W2298532810 date "2016-03-16" @default.
- W2298532810 modified "2023-10-11" @default.
- W2298532810 title "Regulation of Budding Yeast CENP-A levels Prevents Misincorporation at Promoter Nucleosomes and Transcriptional Defects" @default.
- W2298532810 cites W1521067743 @default.
- W2298532810 cites W1547834895 @default.
- W2298532810 cites W1762920546 @default.
- W2298532810 cites W1902128953 @default.
- W2298532810 cites W1966072209 @default.
- W2298532810 cites W1967343937 @default.
- W2298532810 cites W1972245437 @default.
- W2298532810 cites W1972736799 @default.
- W2298532810 cites W1974227032 @default.
- W2298532810 cites W1976242556 @default.
- W2298532810 cites W1978411151 @default.
- W2298532810 cites W1978696228 @default.
- W2298532810 cites W1980689264 @default.
- W2298532810 cites W1981045115 @default.
- W2298532810 cites W1983415836 @default.
- W2298532810 cites W1984708728 @default.
- W2298532810 cites W1992327054 @default.
- W2298532810 cites W1992344689 @default.
- W2298532810 cites W1992906999 @default.
- W2298532810 cites W1993935814 @default.
- W2298532810 cites W1995275121 @default.
- W2298532810 cites W1995668347 @default.
- W2298532810 cites W1995967793 @default.
- W2298532810 cites W2003345607 @default.
- W2298532810 cites W2008675991 @default.
- W2298532810 cites W2011372713 @default.
- W2298532810 cites W2014270114 @default.
- W2298532810 cites W2015697863 @default.
- W2298532810 cites W2019180618 @default.
- W2298532810 cites W2020144427 @default.
- W2298532810 cites W2020599848 @default.
- W2298532810 cites W2021341670 @default.
- W2298532810 cites W2026708487 @default.
- W2298532810 cites W2029388127 @default.
- W2298532810 cites W2031508853 @default.
- W2298532810 cites W2039564969 @default.
- W2298532810 cites W2041002792 @default.
- W2298532810 cites W2043940450 @default.
- W2298532810 cites W2057018194 @default.
- W2298532810 cites W2058368225 @default.
- W2298532810 cites W2059369082 @default.
- W2298532810 cites W2059907545 @default.
- W2298532810 cites W2060141490 @default.
- W2298532810 cites W2063679723 @default.
- W2298532810 cites W2063879491 @default.
- W2298532810 cites W2068608369 @default.
- W2298532810 cites W2069721588 @default.
- W2298532810 cites W2074908472 @default.
- W2298532810 cites W2076095878 @default.
- W2298532810 cites W2079047745 @default.
- W2298532810 cites W2080457024 @default.
- W2298532810 cites W2080466240 @default.
- W2298532810 cites W2084509375 @default.
- W2298532810 cites W2084908610 @default.
- W2298532810 cites W2087576311 @default.
- W2298532810 cites W2087712265 @default.
- W2298532810 cites W2089522966 @default.
- W2298532810 cites W2092604981 @default.
- W2298532810 cites W2095111919 @default.
- W2298532810 cites W2095881412 @default.
- W2298532810 cites W2097065948 @default.
- W2298532810 cites W2097896588 @default.
- W2298532810 cites W2099143842 @default.
- W2298532810 cites W2101654493 @default.
- W2298532810 cites W2103089522 @default.
- W2298532810 cites W2103111663 @default.
- W2298532810 cites W2103441770 @default.
- W2298532810 cites W2108234281 @default.
- W2298532810 cites W2108819945 @default.
- W2298532810 cites W2113478190 @default.
- W2298532810 cites W2114104545 @default.
- W2298532810 cites W2117131162 @default.
- W2298532810 cites W2117219508 @default.
- W2298532810 cites W2119010491 @default.
- W2298532810 cites W2119298573 @default.
- W2298532810 cites W2121989845 @default.
- W2298532810 cites W2123712380 @default.
- W2298532810 cites W2127079829 @default.
- W2298532810 cites W2127879691 @default.
- W2298532810 cites W2131478115 @default.
- W2298532810 cites W2134526812 @default.
- W2298532810 cites W2136623765 @default.
- W2298532810 cites W2137435947 @default.
- W2298532810 cites W2139238510 @default.
- W2298532810 cites W2140457915 @default.
- W2298532810 cites W2142358355 @default.
- W2298532810 cites W2144533535 @default.
- W2298532810 cites W2144792857 @default.
- W2298532810 cites W2146956659 @default.
- W2298532810 cites W2147882992 @default.
- W2298532810 cites W2151766679 @default.