FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2298815274> ?p ?o ?g. }

• W2298815274 endingPage "2486" @default.
• W2298815274 startingPage "2481" @default.
• W2298815274 abstract "Protease sensors for point-of-care testing (POCT) require simple operation, a detection period of less than 20 minutes, and a detection limit of less than 1 ng mL(-1). However, it is difficult to meet these requirements with protease sensors that are based on proteolytic cleavage. This paper reports a highly reproducible protease sensor that allows the sensitive and simple electrochemical detection of the botulinum neurotoxin type E light chain (BoNT/E-LC), which is obtained using (i) low nonspecific adsorption, (ii) high signal-to-background ratio, and (iii) one-step solution treatment. The BoNT/E-LC detection is based on two-step proteolytic cleavage using BoNT/E-LC (endopeptidase) and l-leucine-aminopeptidase (LAP, exopeptidase). Indium-tin oxide (ITO) electrodes are modified partially with reduced graphene oxide (rGO) to increase their electrocatalytic activities. Avidin is then adsorbed on the electrodes to minimize the nonspecific adsorption of proteases. Low nonspecific adsorption allows a highly reproducible sensor response. Electrochemical-chemical (EC) redox cycling involving p-aminophenol (AP) and dithiothreitol (DTT) is performed to obtain a high signal-to-background ratio. After adding a C-terminally AP-labeled oligopeptide, DTT, and LAP simultaneously to a sample solution, no further treatment of the solution is necessary during detection. The detection limits of BoNT/E-LC in phosphate-buffered saline are 0.1 ng mL(-1) for an incubation period of 15 min and 5 fg mL(-1) for an incubation period of 4 h. The detection limit in commercial bottled water is 1 ng mL(-1) for an incubation period of 15 min. The developed sensor is selective to BoNT/E-LC among the four types of BoNTs tested. These results indicate that the protease sensor meets the requirements for POCT." @default.
• W2298815274 created "2016-06-24" @default.
• W2298815274 creator A5012934386 @default.
• W2298815274 creator A5027009676 @default.
• W2298815274 creator A5030364007 @default.
• W2298815274 creator A5075509015 @default.
• W2298815274 creator A5090892663 @default.
• W2298815274 date "2016-01-01" @default.
• W2298815274 modified "2023-10-14" @default.
• W2298815274 title "A highly sensitive and simply operated protease sensor toward point-of-care testing" @default.
• W2298815274 cites W1930649843 @default.
• W2298815274 cites W1957722811 @default.
• W2298815274 cites W1980332132 @default.
• W2298815274 cites W1988508263 @default.
• W2298815274 cites W1988714797 @default.
• W2298815274 cites W2003082566 @default.
• W2298815274 cites W2018195575 @default.
• W2298815274 cites W2018608826 @default.
• W2298815274 cites W2020159277 @default.
• W2298815274 cites W2026634197 @default.
• W2298815274 cites W2035889642 @default.
• W2298815274 cites W2038052393 @default.
• W2298815274 cites W2042181447 @default.
• W2298815274 cites W2066832585 @default.
• W2298815274 cites W2073577134 @default.
• W2298815274 cites W208110057 @default.
• W2298815274 cites W2084532378 @default.
• W2298815274 cites W2102628698 @default.
• W2298815274 cites W2112923194 @default.
• W2298815274 cites W2122977798 @default.
• W2298815274 cites W2133185266 @default.
• W2298815274 cites W2150309756 @default.
• W2298815274 cites W2168525631 @default.
• W2298815274 cites W2223184732 @default.
• W2298815274 cites W2319003941 @default.
• W2298815274 cites W2324836477 @default.
• W2298815274 cites W2326139031 @default.
• W2298815274 cites W2328540890 @default.
• W2298815274 cites W2332492671 @default.
• W2298815274 cites W2334350341 @default.
• W2298815274 cites W289220565 @default.
• W2298815274 cites W4232362978 @default.
• W2298815274 doi "https://doi.org/10.1039/c6an00251j" @default.
• W2298815274 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/26980003" @default.
• W2298815274 hasPublicationYear "2016" @default.
• W2298815274 type Work @default.
• W2298815274 sameAs 2298815274 @default.
• W2298815274 citedByCount "15" @default.
• W2298815274 countsByYear W22988152742016 @default.
• W2298815274 countsByYear W22988152742017 @default.
• W2298815274 countsByYear W22988152742019 @default.
• W2298815274 countsByYear W22988152742020 @default.
• W2298815274 countsByYear W22988152742022 @default.
• W2298815274 crossrefType "journal-article" @default.
• W2298815274 hasAuthorship W2298815274A5012934386 @default.
• W2298815274 hasAuthorship W2298815274A5027009676 @default.
• W2298815274 hasAuthorship W2298815274A5030364007 @default.
• W2298815274 hasAuthorship W2298815274A5075509015 @default.
• W2298815274 hasAuthorship W2298815274A5090892663 @default.
• W2298815274 hasConcept C119128265 @default.
• W2298815274 hasConcept C147789679 @default.
• W2298815274 hasConcept C150394285 @default.
• W2298815274 hasConcept C17525397 @default.
• W2298815274 hasConcept C178790620 @default.
• W2298815274 hasConcept C181199279 @default.
• W2298815274 hasConcept C185592680 @default.
• W2298815274 hasConcept C188095982 @default.
• W2298815274 hasConcept C2776714187 @default.
• W2298815274 hasConcept C2777824588 @default.
• W2298815274 hasConcept C40875361 @default.
• W2298815274 hasConcept C43617362 @default.
• W2298815274 hasConcept C52859227 @default.
• W2298815274 hasConcept C55493867 @default.
• W2298815274 hasConceptScore W2298815274C119128265 @default.
• W2298815274 hasConceptScore W2298815274C147789679 @default.
• W2298815274 hasConceptScore W2298815274C150394285 @default.
• W2298815274 hasConceptScore W2298815274C17525397 @default.
• W2298815274 hasConceptScore W2298815274C178790620 @default.
• W2298815274 hasConceptScore W2298815274C181199279 @default.
• W2298815274 hasConceptScore W2298815274C185592680 @default.
• W2298815274 hasConceptScore W2298815274C188095982 @default.
• W2298815274 hasConceptScore W2298815274C2776714187 @default.
• W2298815274 hasConceptScore W2298815274C2777824588 @default.
• W2298815274 hasConceptScore W2298815274C40875361 @default.
• W2298815274 hasConceptScore W2298815274C43617362 @default.
• W2298815274 hasConceptScore W2298815274C52859227 @default.
• W2298815274 hasConceptScore W2298815274C55493867 @default.
• W2298815274 hasFunder F4320322030 @default.
• W2298815274 hasFunder F4320322120 @default.
• W2298815274 hasIssue "8" @default.
• W2298815274 hasLocation W22988152741 @default.
• W2298815274 hasLocation W22988152742 @default.
• W2298815274 hasOpenAccess W2298815274 @default.
• W2298815274 hasPrimaryLocation W22988152741 @default.
• W2298815274 hasRelatedWork W2017681005 @default.
• W2298815274 hasRelatedWork W2022489431 @default.
• W2298815274 hasRelatedWork W2070737369 @default.
• W2298815274 hasRelatedWork W2775091100 @default.

• next