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- W2299575233 abstract "Background: Bile acid aspiration has been shown to be prevalent among patients with advanced lung disease, which raises the concern that recurrent microaspiration of bile acids may cause lung injury. objectives: To investigate the possible links between bile acids and lung injury using primary bronchial epithelial cell,immortalized human bronchial epithelial cell line (BEAS- 2B) and Human lung carcinoma cells (NCI-H292) cells. Methods: (PBECs), (BEAS- 2B) and (NCI-H292) were cultured. The effect of individual primary and secondary bile acids were evaluated by 48 hour challenge. Post-challenge IL-8 and IL-6 concentrations were measured using commercial ELISAs. The viability of the cells were measured using the Cell Titerblue assay and MTT assays. Results: (PBECs), (BEAS- 2B) and (NCI-H292) cells can be cultured successfully. The acid lithocholic acid was successfully used to stimulate cultured PBECs at different concentrations from 1μmol/l to 20μmol/l. A concentration of lithocholic acid above 18μmol/l causes 100% PBEC death. Potentially physiological challenges with bile acids led to release of IL-8 from lung transplant PBECs and (BEAS-2B). (NCI-H292) cells were stimulated with lithocholic acid and Deoxycholic acid. Lithocholic acid above 20μmol/l caused 100% cell death wheras Deoxycholic acid above 50μmol/l caused 50% cell death. (BEAS-2B) were stimulated with lithocholic acid, Deoxycholic acid and Chenodeoxycholic acid. Lithocholic acid above 20μmol/l caused 95% cell death wheras Deoxycholic acid above 75μmol/l caused 20% cell death. Chenodeoxycholic acid above 50 μmol/l caused 10% cell death. Conclusion: Aspiration of bile acids may cause cell damage, inflammation and cell death." @default.
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- W2299575233 date "2015-09-01" @default.
- W2299575233 modified "2023-09-27" @default.
- W2299575233 title "Aspiration, epithelial injury and lung diseases" @default.
- W2299575233 doi "https://doi.org/10.1183/13993003.congress-2015.pa879" @default.
- W2299575233 hasPublicationYear "2015" @default.
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