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- W2299875480 endingPage "454" @default.
- W2299875480 startingPage "445" @default.
- W2299875480 abstract "Abstract This work proposed a label‐free, cost‐effective and fairly sensitive electrogenerated chemiluminescence (ECL) strategy for the specific detection of lysozyme based on the hemin/G‐quadruplex hybrid. Gold nanoparticles were spread onto the chitosan thin‐film as substrate to adsorb thiolated captures: a hairpin‐structured DNA, integrating dual head‐tail connected functional domains: one aptamer sequence for lysozyme and the other for hemin (iron(III) proto ‐porphyrin IX). In the presence of the both, the hairpin conformation unfolded and transformed into the hemin/G‐quadruplex motif, which quenched the ECL emission of underlaid quantum dots significantly via the consumption of dissolved oxygen as endogenous coreactant. This construction enabled a wide linear response to lysozyme, ranging from 20 pg·mL −1 to 5 µg·mL −1 , with a detection limit as low as 4.95 pg·mL −1 ( e.g. 9.4 pmol·L −1 ), demonstrating the prospective utilization of DNA technologies in bioanalysis." @default.
- W2299875480 created "2016-06-24" @default.
- W2299875480 creator A5011643475 @default.
- W2299875480 creator A5013391420 @default.
- W2299875480 date "2016-03-08" @default.
- W2299875480 modified "2023-10-07" @default.
- W2299875480 title "State-of-the-Art in Electrophilic Trifluoromethylthiolation Reagents" @default.
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