Matches in SemOpenAlex for { <https://semopenalex.org/work/W2299972938> ?p ?o ?g. }
Showing items 1 to 86 of
86
with 100 items per page.
- W2299972938 endingPage "117" @default.
- W2299972938 startingPage "114" @default.
- W2299972938 abstract "We have previously reported that the application of sphingomyelin-based liposomes (SM-L) to a three-dimensional cultured skin model increase the content of ceramides NS, NP, AS and AP. However, the mechanism responsible for these increased ceramide levels was not identified.SM-L and sphingomyelinase (SMase) were combined and incubated at 37 °C for 24 h. SM-L were also applied to three-dimensional cultured skin for 24 h and quantification of SMase and β-glucocerebrosidase (β-GCase) mRNA expression levels performed using real-time PCR. Additionally, three dimensional cultured skin was incubated with SM-L and the β-GCase inhibitor conduritol B epoxide (CBE) and the ceramide content determined by high performance thin layer chromatography.We observed generation of ceramide NS after reaction of SM-L and SMase. However, the other ceramide classes were not detected. Notably, SMase and β-GCase mRNA expression levels were significantly increased in cells of the skin model following application of SM-L. The levels of ceramides NS, NP, AS and AP were decreased by treatment with CBE. However, only ceramide NS was significantly increased by treatment with CBE and SM-L in combination.These findings indicate that application of SM-L to cultured skin upregulates the expression of SMase and β-GCase and increases ceramide content." @default.
- W2299972938 created "2016-06-24" @default.
- W2299972938 creator A5030332751 @default.
- W2299972938 creator A5031835122 @default.
- W2299972938 date "2016-04-01" @default.
- W2299972938 modified "2023-09-27" @default.
- W2299972938 title "Upregulation of gene expression levels of ceramide metabolic enzymes after application of sphingomyelin-based liposomes to a three-dimensional cultured human epidermis model" @default.
- W2299972938 cites W1975986537 @default.
- W2299972938 cites W1990084557 @default.
- W2299972938 cites W2005153646 @default.
- W2299972938 cites W2007993998 @default.
- W2299972938 cites W2026275425 @default.
- W2299972938 cites W2031317659 @default.
- W2299972938 cites W2035411151 @default.
- W2299972938 cites W2035660063 @default.
- W2299972938 cites W2050548618 @default.
- W2299972938 cites W2066929985 @default.
- W2299972938 cites W2067017343 @default.
- W2299972938 cites W2070935457 @default.
- W2299972938 cites W2106246921 @default.
- W2299972938 cites W2117193543 @default.
- W2299972938 cites W2136623004 @default.
- W2299972938 cites W2152311269 @default.
- W2299972938 cites W2158612420 @default.
- W2299972938 doi "https://doi.org/10.1016/j.bbrc.2016.03.061" @default.
- W2299972938 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/26995090" @default.
- W2299972938 hasPublicationYear "2016" @default.
- W2299972938 type Work @default.
- W2299972938 sameAs 2299972938 @default.
- W2299972938 citedByCount "1" @default.
- W2299972938 countsByYear W22999729382019 @default.
- W2299972938 crossrefType "journal-article" @default.
- W2299972938 hasAuthorship W2299972938A5030332751 @default.
- W2299972938 hasAuthorship W2299972938A5031835122 @default.
- W2299972938 hasBestOaLocation W22999729382 @default.
- W2299972938 hasConcept C104317684 @default.
- W2299972938 hasConcept C127561419 @default.
- W2299972938 hasConcept C153911025 @default.
- W2299972938 hasConcept C181199279 @default.
- W2299972938 hasConcept C185154212 @default.
- W2299972938 hasConcept C185592680 @default.
- W2299972938 hasConcept C190283241 @default.
- W2299972938 hasConcept C2777851122 @default.
- W2299972938 hasConcept C2781070647 @default.
- W2299972938 hasConcept C41625074 @default.
- W2299972938 hasConcept C55493867 @default.
- W2299972938 hasConcept C61322309 @default.
- W2299972938 hasConcept C61705674 @default.
- W2299972938 hasConcept C86803240 @default.
- W2299972938 hasConceptScore W2299972938C104317684 @default.
- W2299972938 hasConceptScore W2299972938C127561419 @default.
- W2299972938 hasConceptScore W2299972938C153911025 @default.
- W2299972938 hasConceptScore W2299972938C181199279 @default.
- W2299972938 hasConceptScore W2299972938C185154212 @default.
- W2299972938 hasConceptScore W2299972938C185592680 @default.
- W2299972938 hasConceptScore W2299972938C190283241 @default.
- W2299972938 hasConceptScore W2299972938C2777851122 @default.
- W2299972938 hasConceptScore W2299972938C2781070647 @default.
- W2299972938 hasConceptScore W2299972938C41625074 @default.
- W2299972938 hasConceptScore W2299972938C55493867 @default.
- W2299972938 hasConceptScore W2299972938C61322309 @default.
- W2299972938 hasConceptScore W2299972938C61705674 @default.
- W2299972938 hasConceptScore W2299972938C86803240 @default.
- W2299972938 hasIssue "1" @default.
- W2299972938 hasLocation W22999729381 @default.
- W2299972938 hasLocation W22999729382 @default.
- W2299972938 hasLocation W22999729383 @default.
- W2299972938 hasOpenAccess W2299972938 @default.
- W2299972938 hasPrimaryLocation W22999729381 @default.
- W2299972938 hasRelatedWork W1044512906 @default.
- W2299972938 hasRelatedWork W2000823512 @default.
- W2299972938 hasRelatedWork W2009988896 @default.
- W2299972938 hasRelatedWork W2033859595 @default.
- W2299972938 hasRelatedWork W2034886683 @default.
- W2299972938 hasRelatedWork W2037430500 @default.
- W2299972938 hasRelatedWork W2041922728 @default.
- W2299972938 hasRelatedWork W2063653985 @default.
- W2299972938 hasRelatedWork W2085982495 @default.
- W2299972938 hasRelatedWork W2464951831 @default.
- W2299972938 hasVolume "473" @default.
- W2299972938 isParatext "false" @default.
- W2299972938 isRetracted "false" @default.
- W2299972938 magId "2299972938" @default.
- W2299972938 workType "article" @default.