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- W2300107652 abstract "目的帕金森病（Parkinson’s disease,PD）是常见的神经退行性疾病,儿茶酚异喹啉类神经毒素（salsolinol,Sal）作为内源性神经毒素是其可能的致病因素,而S-腺苷甲硫氨酸（S-adenosyl methionine,SAM）目前在帕金森病的治疗上有应用的可能,所以有必要对其在体内的作用进行研究。方法本实验采用体外培养的多巴胺能细胞SH-SY5Y,首先使用MTT的方法对Sal以及SAM与Sal共同诱导后细胞存活率进行研究,使用了Hoechst 33258染色的方式检测凋亡,并检测ATP,最后使用高效液相色谱偶联电化学检测器（high performance liquid chromatography couple electrochemical detector,HPLC-ECD）检测了Sal和SAM代谢生成的氮甲基去甲猪毛菜碱（N-methylsalsolinol,NMSal）在细胞中的浓度。结果 SAM可以促进Sal引起的细胞存活率的降低,同时凋亡水平升高,ATP浓度降低,而且SAM诱导之后,Sal代谢生成的NMSal浓度也随之升高。结论这些结果说明了SAM会促进Sal在多巴胺能神经细胞中的毒性作用,因此SAM可能不利于PD的治疗。" @default.
- W2300107652 created "2016-06-24" @default.
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- W2300107652 date "2015-01-01" @default.
- W2300107652 modified "2023-09-28" @default.
- W2300107652 title "S-腺苷甲硫氨酸增加salsolinol对多巴胺能神经细胞SH-SY5Y的毒性" @default.
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