Matches in SemOpenAlex for { <https://semopenalex.org/work/W2300462316> ?p ?o ?g. }
Showing items 1 to 72 of
72
with 100 items per page.
- W2300462316 abstract "Ferroportin-1 (Fpn1) is a highly conserved 571-amino acid protein with the human, mouse, and rat polypeptides having 90–95% sequence identity at the amino acid level. Disruption of Fpn1 expression in zebrafish and mice results in embryonic lethality, while conditional knockout of the gene encoding Fpn1 in the intestine at the postnatal stage leads to severe iron deficiency anemia and iron accumulation in duodenal enterocytes. These studies suggest that Fpn1 is the major, if not the sole, exporter protein for iron. Export of dietary iron is thought to be facilitated by hephaestin (Hpn), a multicopper ferroxidase in the basolateral membrane of duodenal enterocytes. However, the precise mechanism of iron transport by Fpn1 and Hpn remains to be elucidated.The absence of an iron export mechanism in Saccharomyces cerevisiae was utilized to explore how Fpn1 and Hpn function in iron export. Unlike humans, S. cerevisiae transports excess iron into a storage vacuole through the vacuolar iron transporter, Ccc1p. The Δccc1 mutant fails to store excess iron; as a result, iron accumulates in the cytosol and cells die due to oxidative stress when exposed to high concentrations of extracellular iron. By expressing recombinant human Fpn1 and Hpn in S. cerevisiae, an iron export system was introduced. The functionality of rhHpn in S. cerevisiae was confirmed by both a ferrozine ferroxidase assay and a transferrin iron-loading assay. The effects of the individual or both recombinant proteins on iron sensitivity of the Δccc1 yeast with an additional fet3 (the yeast homolog of Hpn) deletion were evaluated. Recombinant human Fpn1 and Hpn suppressed the lethal phenotype of the Δccc1 mutant while co-expression of rhHpn with rhFpn1 led to a stronger rescue phenotype of the Δccc1 mutant under concentrations of high extracellular iron. The expression of rhFpn1 and / or rhHpn also relieved the copper sensitive Δfet3 mutant from copper stress. A physical interaction between rhFpn1 and rhHpn was demonstrated by cross-linking with BS³. These studies advance our knowledge of the roles Fpn1 and Hpn play in human enterocytes and suggest that these proteins are intimately involved in iron export from enterocytes into the blood." @default.
- W2300462316 created "2016-06-24" @default.
- W2300462316 creator A5084640495 @default.
- W2300462316 date "2010-01-01" @default.
- W2300462316 modified "2023-09-23" @default.
- W2300462316 title "Studies on the roles of human ferroportin and hephaestin in iron homeostasis" @default.
- W2300462316 doi "https://doi.org/10.14288/1.0071383" @default.
- W2300462316 hasPublicationYear "2010" @default.
- W2300462316 type Work @default.
- W2300462316 sameAs 2300462316 @default.
- W2300462316 citedByCount "0" @default.
- W2300462316 crossrefType "journal-article" @default.
- W2300462316 hasAuthorship W2300462316A5084640495 @default.
- W2300462316 hasConcept C104317684 @default.
- W2300462316 hasConcept C143065580 @default.
- W2300462316 hasConcept C149011108 @default.
- W2300462316 hasConcept C185592680 @default.
- W2300462316 hasConcept C22814914 @default.
- W2300462316 hasConcept C2776531687 @default.
- W2300462316 hasConcept C2776710925 @default.
- W2300462316 hasConcept C2777576037 @default.
- W2300462316 hasConcept C2779222958 @default.
- W2300462316 hasConcept C28452662 @default.
- W2300462316 hasConcept C2911035656 @default.
- W2300462316 hasConcept C32264632 @default.
- W2300462316 hasConcept C55493867 @default.
- W2300462316 hasConcept C62231903 @default.
- W2300462316 hasConcept C86803240 @default.
- W2300462316 hasConcept C95444343 @default.
- W2300462316 hasConceptScore W2300462316C104317684 @default.
- W2300462316 hasConceptScore W2300462316C143065580 @default.
- W2300462316 hasConceptScore W2300462316C149011108 @default.
- W2300462316 hasConceptScore W2300462316C185592680 @default.
- W2300462316 hasConceptScore W2300462316C22814914 @default.
- W2300462316 hasConceptScore W2300462316C2776531687 @default.
- W2300462316 hasConceptScore W2300462316C2776710925 @default.
- W2300462316 hasConceptScore W2300462316C2777576037 @default.
- W2300462316 hasConceptScore W2300462316C2779222958 @default.
- W2300462316 hasConceptScore W2300462316C28452662 @default.
- W2300462316 hasConceptScore W2300462316C2911035656 @default.
- W2300462316 hasConceptScore W2300462316C32264632 @default.
- W2300462316 hasConceptScore W2300462316C55493867 @default.
- W2300462316 hasConceptScore W2300462316C62231903 @default.
- W2300462316 hasConceptScore W2300462316C86803240 @default.
- W2300462316 hasConceptScore W2300462316C95444343 @default.
- W2300462316 hasLocation W23004623161 @default.
- W2300462316 hasOpenAccess W2300462316 @default.
- W2300462316 hasPrimaryLocation W23004623161 @default.
- W2300462316 hasRelatedWork W1550150722 @default.
- W2300462316 hasRelatedWork W1849807599 @default.
- W2300462316 hasRelatedWork W1968898079 @default.
- W2300462316 hasRelatedWork W1970761716 @default.
- W2300462316 hasRelatedWork W1974015253 @default.
- W2300462316 hasRelatedWork W1982245868 @default.
- W2300462316 hasRelatedWork W1992892611 @default.
- W2300462316 hasRelatedWork W1997777936 @default.
- W2300462316 hasRelatedWork W2002390714 @default.
- W2300462316 hasRelatedWork W2029012493 @default.
- W2300462316 hasRelatedWork W2029473695 @default.
- W2300462316 hasRelatedWork W2046440396 @default.
- W2300462316 hasRelatedWork W2046605546 @default.
- W2300462316 hasRelatedWork W2051591863 @default.
- W2300462316 hasRelatedWork W2073461422 @default.
- W2300462316 hasRelatedWork W2077667351 @default.
- W2300462316 hasRelatedWork W2796417179 @default.
- W2300462316 hasRelatedWork W2974558407 @default.
- W2300462316 hasRelatedWork W3014802319 @default.
- W2300462316 hasRelatedWork W3184190931 @default.
- W2300462316 isParatext "false" @default.
- W2300462316 isRetracted "false" @default.
- W2300462316 magId "2300462316" @default.
- W2300462316 workType "article" @default.