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- W2300486253 abstract "Abstract Effects associated with aflatoxins (AFs), principally aflatoxin B 1 (AFB 1 ) have necessitated strategies to eliminate their occurrence in commodities along the food chain. This study therefore, investigated the AFB 1 biodegradation ability of Staphylococcus warneri , Sporosarcina sp. and Lysinibacillus fusiformis liquid cultures and cell lysates (disrupted in the presence or absence of protease inhibitors to obtain lysates). These were incubated with AFB 1 (2.5 μg/mL) for 3, 6, 12, 24 and 48 h. AFB 1 degradation was subsequently monitored on high performance liquid chromatography (HPLC) and results indicated that after 48 h, % AFB 1 degradation by the liquid cultures of Lysinibacillus fusisormis, S. warneri and Sporosarcina sp. were 61.3, 47.7 and 46.9%, respectively. After 12 h of incubation, a 100% AFB 1 degradation was observed for all protease inhibited lysates tested. To establish toxicity of the AFB 1 biotransformed products, results from a cytotoxicity study against human lymphocytes demonstrated that the products exhibited significantly (p ≤ 0.05) lower cytotoxic effect compared to the parent AFB 1 . From this study, it can be deduced that the mechanism of AFB 1 degradation was enzymatic and that protease inhibition of cells before disruption, could increase this enzymatic activity. Conclusively, the potential of these lysates as a biotechnological approach towards decontaminating AFB 1 is promising." @default.
- W2300486253 created "2016-06-24" @default.
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- W2300486253 date "2016-10-01" @default.
- W2300486253 modified "2023-10-13" @default.
- W2300486253 title "Degradation and detoxification of AFB1 by Staphylocococcus warneri, Sporosarcina sp. and Lysinibacillus fusiformis" @default.
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- W2300486253 doi "https://doi.org/10.1016/j.foodcont.2016.03.021" @default.
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