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• W2300621655 endingPage "259" @default.
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• W2300621655 abstract "A well-organized immobilization of bio-receptors is a crucial goal in biosensing, especially to achieve high reproducibility, sensitivity and specificity. These requirements are usually attained with a controlled chemical/biochemical functionalization that creates a stable layer on a sensor surface. In this work, a chemical modification protocol for silicon-based surfaces to be applied in biosensing devices is presented. An anhydrous silanization step through 3-aminopropylsilane (APTES), followed by a further derivatization with succinic anhydride (SA), is optimized to generate an ordered flat layer of carboxylic groups. The properties of APTES/SA modified surface were compared with a functionalization in which glutaraldehyde (GA) is used as crosslinker instead of SA, in order to have a comparison with an established and largely applied procedure. Moreover, a functionalization based on the controlled deposition of a plasma polymerized acrylic acid (PPAA) thin film was used as a reference for carboxylic reactivity. Advantages and drawbacks of the considered methods are highlighted, through physico-chemical characterizations (OCA, XPS, and AFM) and by means of a functional Protein G/Antibody immunoassay. These analyses reveal that the most homogeneous, reproducible and active surface is achieved by using the optimized APTES/SA coupling." @default.
• W2300621655 created "2016-06-24" @default.
• W2300621655 creator A5000548212 @default.
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• W2300621655 date "2016-07-01" @default.
• W2300621655 modified "2023-09-27" @default.
• W2300621655 title "Optimization and characterization of a homogeneous carboxylic surface functionalization for silicon-based biosensing" @default.
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• W2300621655 doi "https://doi.org/10.1016/j.colsurfb.2016.03.048" @default.
• W2300621655 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/27022864" @default.
• W2300621655 hasPublicationYear "2016" @default.
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