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- W2300685024 abstract "TKU024，係以烏賊軟骨粉末為唯一碳/氮源，篩選自台灣中部土壤之一株幾丁聚醣酶生產菌，經鑑定為Acinetobacter calcoaceticus。TKU024 幾丁聚醣酶較適生產條件為1.5% 烏賊軟骨粉末、0.1% K2HPO4、0.05% MgSO4．7H2O之液態培養基(pH 8)於37℃振盪培養4 天。將發酵所得上清液離心，經硫酸銨沉澱、DEAE-Sepharose、Mcaro-Prap DEAE 及Phenyl Sepharose 管柱層析後，可純化出兩個幾丁聚醣酶(C1/C2)，經SDS-PAGE 測得分子量分別為27 kDa及66 kDa。經胜肽質譜鑑定，C1 分別與Cellvibrio japonicus Ueda107 之幾丁質酶(GenBank 編號為gi1192361966)、Pseudomonas aeruginosa PA01 之琥珀脫氫酶鐵硫亞基(GenBank 編號為gi115596781)相似，相似度分別為4%及10%；C2與 Arthrobacter sp. 之幾丁質酶(GenBank 編號為gi127065204)相似，相似度為7%。於最適反應pH、最適反應溫度、pH 安定性、熱安定性方面，C1為pH 6、50℃、pH 8-9 及＜70℃，C2 為pH 7、60℃、pH 6 及＜60℃。C1 活性受5 mM Cu2+、Zn2+、Mn2+及2% (v/v) SDS 抑制，且於4~5 mM Mn2+完全抑制；C2 活性受5 mM Ca2+、Urea、EDTA、Zn2+、Mn2+抑制，但在1 mM Mn2+則有少許促進效果，於2% (v/v) SDS、Triton X-100、Tween 20 和0.5% (v/v)Tween 20 有輕微抑制效果。" @default.
- W2300685024 created "2016-06-24" @default.
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- W2300685024 date "2010-01-01" @default.
- W2300685024 modified "2023-09-23" @default.
- W2300685024 title "Acinetobacter calcoaceticus TKU024 所生產幾丁聚醣酶之特性分析與應用" @default.
- W2300685024 doi "https://doi.org/10.6846/tku.2010.00936" @default.
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