Matches in SemOpenAlex for { <https://semopenalex.org/work/W230085868> ?p ?o ?g. }
Showing items 1 to 86 of
86
with 100 items per page.
- W230085868 abstract "Tibial dyschondroplasia (TD) is a metabolic cartilage disease that causes major leg problems in poultry. We have developed a model to induce TD at high incidence and severity by feeding week-old broiler chickens diets containing 50-100 ppm thiram for two days that is amenable to study the mechanisms of the disease and screen for factors that may prevent it. The objectives for this study were to understand what causes avascularity of TD lesions and to determine if certain micronutrients added to their basal diets may protect the chickens from this disease. Week-old broiler chickens were fed 100 ppm thiram and monitored for the expression of selective genes associated with vascularization and cell survival at 48 and 166 h after feeding thiram. RT-PCR and capillary electrophoresis was used to determine the expression of vascular endothelial growth factor (VEGF), its receptors (VEGFR), and anti-apoptotic protein BCL-2 genes. The results showed maximal suppression of VEGFR and BCL-2 genes at 48 h following thiram feeding, when only sporadic capillary endothelial cell death was observed by histochemical methods. Except for BCL-2 these suppressions were not evident at 166 h when the lesions are visually discernible with chondrocyte death apparent histologically and histochemically. Further analysis suggested that the dead cells in the lesions were those affected early during thiram treatment. The avascularity of the TD lesion seems to be related to the early death of capillary endothelial cells which express VEGFR through which VEGF, produced by hypertrophic chondrocytes, transduce signals for angiogenesis. The thiram-initiated cell death was also evident by the suppression of BCL-2 gene. In order to test the potential of this model as a bioassay for nutrients that may protect birds against TD, selective micronutrients were added to the diets and the birds were subjected to TD-induction with 50 ppm thiram and examined for disease incidence and severity on day 15. Of the tested dietary supplements that included vitamin A, folic acid, trace minerals, and different forms of vitamin D, only folic acid at 5 times its NRC recommended concentration protected chickens against TD. These studies provide an example of how thiram-induced TD can be exploited to study the mechanisms of this economically important poultry metabolic disease. ______________________________________________________________________________" @default.
- W230085868 created "2016-06-24" @default.
- W230085868 creator A5003660740 @default.
- W230085868 creator A5040413241 @default.
- W230085868 creator A5059212395 @default.
- W230085868 date "2006-01-01" @default.
- W230085868 modified "2023-09-24" @default.
- W230085868 title "Thiram-induced tibial dyschondroplasia: a model to study its pathogenesis and prevention." @default.
- W230085868 cites W1573363574 @default.
- W230085868 cites W1580513872 @default.
- W230085868 cites W1583699618 @default.
- W230085868 cites W1981259231 @default.
- W230085868 cites W1992043161 @default.
- W230085868 cites W1994435087 @default.
- W230085868 cites W2004706898 @default.
- W230085868 cites W2008503114 @default.
- W230085868 cites W2012390831 @default.
- W230085868 cites W2037967318 @default.
- W230085868 cites W2039666270 @default.
- W230085868 cites W2050659944 @default.
- W230085868 cites W2051737747 @default.
- W230085868 cites W2056218199 @default.
- W230085868 cites W2074611556 @default.
- W230085868 cites W2077129920 @default.
- W230085868 cites W2082383612 @default.
- W230085868 cites W2108903952 @default.
- W230085868 cites W2143669904 @default.
- W230085868 cites W2161053312 @default.
- W230085868 cites W2166322421 @default.
- W230085868 cites W2166433013 @default.
- W230085868 cites W2168887995 @default.
- W230085868 cites W2319746052 @default.
- W230085868 cites W2325381427 @default.
- W230085868 cites W2183492944 @default.
- W230085868 hasPublicationYear "2006" @default.
- W230085868 type Work @default.
- W230085868 sameAs 230085868 @default.
- W230085868 citedByCount "0" @default.
- W230085868 crossrefType "journal-article" @default.
- W230085868 hasAuthorship W230085868A5003660740 @default.
- W230085868 hasAuthorship W230085868A5040413241 @default.
- W230085868 hasAuthorship W230085868A5059212395 @default.
- W230085868 hasConcept C105702510 @default.
- W230085868 hasConcept C123012128 @default.
- W230085868 hasConcept C126322002 @default.
- W230085868 hasConcept C134018914 @default.
- W230085868 hasConcept C142724271 @default.
- W230085868 hasConcept C161176658 @default.
- W230085868 hasConcept C16685009 @default.
- W230085868 hasConcept C167734588 @default.
- W230085868 hasConcept C202751555 @default.
- W230085868 hasConcept C2777025900 @default.
- W230085868 hasConcept C2777855856 @default.
- W230085868 hasConcept C2780394083 @default.
- W230085868 hasConcept C2780550940 @default.
- W230085868 hasConcept C2781403057 @default.
- W230085868 hasConcept C54355233 @default.
- W230085868 hasConcept C6557445 @default.
- W230085868 hasConcept C71924100 @default.
- W230085868 hasConcept C86803240 @default.
- W230085868 hasConceptScore W230085868C105702510 @default.
- W230085868 hasConceptScore W230085868C123012128 @default.
- W230085868 hasConceptScore W230085868C126322002 @default.
- W230085868 hasConceptScore W230085868C134018914 @default.
- W230085868 hasConceptScore W230085868C142724271 @default.
- W230085868 hasConceptScore W230085868C161176658 @default.
- W230085868 hasConceptScore W230085868C16685009 @default.
- W230085868 hasConceptScore W230085868C167734588 @default.
- W230085868 hasConceptScore W230085868C202751555 @default.
- W230085868 hasConceptScore W230085868C2777025900 @default.
- W230085868 hasConceptScore W230085868C2777855856 @default.
- W230085868 hasConceptScore W230085868C2780394083 @default.
- W230085868 hasConceptScore W230085868C2780550940 @default.
- W230085868 hasConceptScore W230085868C2781403057 @default.
- W230085868 hasConceptScore W230085868C54355233 @default.
- W230085868 hasConceptScore W230085868C6557445 @default.
- W230085868 hasConceptScore W230085868C71924100 @default.
- W230085868 hasConceptScore W230085868C86803240 @default.
- W230085868 hasLocation W2300858681 @default.
- W230085868 hasOpenAccess W230085868 @default.
- W230085868 hasPrimaryLocation W2300858681 @default.
- W230085868 hasRelatedWork W2167918906 @default.
- W230085868 isParatext "false" @default.
- W230085868 isRetracted "false" @default.
- W230085868 magId "230085868" @default.
- W230085868 workType "article" @default.