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- W2300911610 abstract "We have developed a capillary polymerase chain reaction (PCR) method to detect both canine parvovirus (CPV) and feline panleukopenia virus (FPLV) as well as to differentiate these two viruses in fecal specimens. The sequences of the PCR primer pair for detection were selected from the conserved region in the VP1/VP2 gene of both viruses. The PCR for differentiation is based on a difference between nucleotide sequences of the capsid protein gene of CPV and FPLV that determines the canine host range of CPV. The result of sensitivity tests demonstrates our PCR assay was 100- to 10,000-fold higher than that of the culture method and the hemagglutination (HA) test. To establish a proper procedure for preparing clinical samples, we compared the results of the PCR assay performed with fifty fecal samples taken from dogs suspected of CPV infection that underwent differnt DNA extraction methods. In addition, these samples were also examined by other standard assays for CPV infection, and the results were compared with this PCR assay. We conclude that this capillary PCR system is a highly specific and sensitive diagnostic method for CPV and FPV infection, which is also potential to monitor the interspecies transmission of these ever changing viruses between dog and cats efficiently." @default.
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- W2300911610 date "2000-03-01" @default.
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- W2300911610 title "Capillary PCR for Detecting and Differentiating Feline Panleukopenia Virus and Canine Parvovirus in Fecal Specimens" @default.
- W2300911610 doi "https://doi.org/10.7009/jtcsvs.200003.0014" @default.
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