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• W2300946019 abstract "Article1 September 1985free access Virus-induced synthesis of messenger RNAs for precursors of pathogenesis-related proteins in tobacco R.A.M. Hooft van Huijsduijnen R.A.M. Hooft van Huijsduijnen Department of Biochemistry, State University of Leiden, Wassenaarseweg 64, The Netherlands Search for more papers by this author B.J.C. Cornelissen B.J.C. Cornelissen Department of Biochemistry, State University of Leiden, Wassenaarseweg 64, The Netherlands Search for more papers by this author L.C. van Loon L.C. van Loon Department of Plant Physiology, Agricultural University, Arboretumlaan 4, 6703 BD Wageningen, The Netherlands Search for more papers by this author J.H. van Boom J.H. van Boom Department of Organic Chemistry, State University of Leiden, Wassenaarseweg 76, 2333 AL Leiden, The Netherlands Search for more papers by this author M. Tromp M. Tromp Department of Organic Chemistry, State University of Leiden, Wassenaarseweg 76, 2333 AL Leiden, The Netherlands Search for more papers by this author J.F. Bol J.F. Bol Department of Biochemistry, State University of Leiden, Wassenaarseweg 64, The Netherlands Search for more papers by this author R.A.M. Hooft van Huijsduijnen R.A.M. Hooft van Huijsduijnen Department of Biochemistry, State University of Leiden, Wassenaarseweg 64, The Netherlands Search for more papers by this author B.J.C. Cornelissen B.J.C. Cornelissen Department of Biochemistry, State University of Leiden, Wassenaarseweg 64, The Netherlands Search for more papers by this author L.C. van Loon L.C. van Loon Department of Plant Physiology, Agricultural University, Arboretumlaan 4, 6703 BD Wageningen, The Netherlands Search for more papers by this author J.H. van Boom J.H. van Boom Department of Organic Chemistry, State University of Leiden, Wassenaarseweg 76, 2333 AL Leiden, The Netherlands Search for more papers by this author M. Tromp M. Tromp Department of Organic Chemistry, State University of Leiden, Wassenaarseweg 76, 2333 AL Leiden, The Netherlands Search for more papers by this author J.F. Bol J.F. Bol Department of Biochemistry, State University of Leiden, Wassenaarseweg 64, The Netherlands Search for more papers by this author Author Information R.A.M. Hooft Huijsduijnen1, B.J.C. Cornelissen1, L.C. Loon3, J.H. Boom2, M. Tromp2 and J.F. Bol1 1Department of Biochemistry, State University of Leiden, Wassenaarseweg 64, The Netherlands 2Department of Organic Chemistry, State University of Leiden, Wassenaarseweg 76, 2333 AL Leiden, The Netherlands 3Department of Plant Physiology, Agricultural University, Arboretumlaan 4, 6703 BD Wageningen, The Netherlands The EMBO Journal (1985)4:2167-2171https://doi.org/10.1002/j.1460-2075.1985.tb03911.x PDFDownload PDF of article text and main figures. ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinked InMendeleyWechatReddit Figures & Info Infection of Samsun NN tobacco with tobacco mosaic virus (TMV) induces a number of host-encoded, so-called pathogenesis-related (PR-) proteins, which are found in the intercellular space of the leaf and are associated with induced resistance. By immunoprecipitation of their in vitro translation products we were able to detect the mRNAs corresponding to a number of PR-proteins in TMV-infected tobacco, but not in healthy plants. Analysis by the Northern blot technique using cloned cDNA of PR1-mRNAs as probe showed that the mRNAs for the closely related proteins PR1a, 1b and 1c occur at a low level in healthy tobacco; upon TMV infection this level is increased >100-fold. The PR1-specific probe did not hybridize to mRNAs corresponding to other PR-proteins. Sequencing of the 5′-terminal region of PR1-mRNAs showed that PR1-proteins are derived from precursors by removal of an N-terminal signal peptide of 30 amino acids. Previous ArticleNext Article Volume 4Issue 91 September 1985In this issue RelatedDetailsLoading ..." @default.
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