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- W2301476057 abstract "Ripe ova and sperm were obtained from Green Sea Urchins (Strongvlocentrotus drochbachiensis) collected from Kasitsna Bay, Alaska, and ova were fertilized in vitro. Embryos were immediately placed in plastic bags secured to floating racks deployed in the bay. The bags were suspended just below the surface of the water and at 1 and 2 meter depths for up to 120 hours. Bags were either left uncovered, covered with Mylar plastic (which blocks out UV-B but not UV-A radiations), or covered with dark plastic. The number of damaged DNA sites was determined by digesting the DNA with enzymes isolated from the bacterium Micrococcus luteus which cleave the DNA at damaged sites. It was found that DNA damage was present in a dose-dependent fashion with the amount of damage in embryos from the uncovered bags > Mylar covered bags > dark covered bags. No dimers were detected from embryos at 1 or 2 m. depths. Also, the number of damaged sites varied from day to day. Finally, the number of damaged sites was positively correlated with percent abnormal embryos in each bag. The results are discussed with relation to monitoring UV-B effects and ecological consequences of enhanced UV-B radiation." @default.
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- W2301476057 date "1995-12-31" @default.
- W2301476057 modified "2023-09-27" @default.
- W2301476057 title "Ultraviolet radiation-specific DNA damage and embryonic viability in sea urchins from Kasitsna Bay, Alaska" @default.
- W2301476057 hasPublicationYear "1995" @default.
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