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- W2301492266 abstract "Mammalian bodies have more than a billion cells per cubic centimeter, which makes whole-body cell (WBC) profiling of an organism one of the ultimate challenges in biology and medicine. Recent advances in tissue-clearing technology have enabled rapid and comprehensive cellular analyses in whole organs and in the whole body by a combination of state-of-the-art technologies of optical imaging and image informatics. In this review, we focus mainly on the chemical principles in currently available techniques for tissue clearing and staining to facilitate our understanding of their underlying mechanisms. Tissue clearing is usually conducted by the following steps: (a) fixation, (b) permeabilization, (c) decolorizing, and (d) refractive index (RI) matching. To phenotype individual cells after tissue clearing, it is important to visualize genetically encoded fluorescent reporters and/or to stain tissues with fluorescent dyes, fluorescent labeled antibodies, or nucleic acid probes. Although some technical challenges remain, the chemical principles in tissue clearing and staining for WBC profiling will enable various applications, such as identifying cellular circuits across multiple organs and measuring their dynamics in stochastic and proliferative cellular processes, for example, autoimmune and malignant neoplastic diseases." @default.
- W2301492266 created "2016-06-24" @default.
- W2301492266 creator A5003599781 @default.
- W2301492266 creator A5012199498 @default.
- W2301492266 creator A5025894459 @default.
- W2301492266 creator A5064606212 @default.
- W2301492266 creator A5082951672 @default.
- W2301492266 date "2016-10-06" @default.
- W2301492266 modified "2023-10-15" @default.
- W2301492266 title "Chemical Principles in Tissue Clearing and Staining Protocols for Whole-Body Cell Profiling" @default.
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