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• W2301517287 abstract "The opportunistic pathogen Pseudomonas aeruginosa causes a wide range of infections in multiple hosts by releasing an arsenal of virulence factors such as pyocyanin. Despite numerous reports on the pleiotropic cellular targets of pyocyanin toxicity in vivo, its impact on erythrocytes remains elusive. Erythrocytes undergo an apoptosis-like cell death called eryptosis which is characterized by cell shrinkage and phosphatidylserine (PS) externalization; this process confers a procoagulant phenotype on erythrocytes as well as fosters their phagocytosis and subsequent clearance from the circulation. Herein, we demonstrate that P. aeruginosa pyocyanin-elicited PS exposure and cell shrinkage in erythrocyte while preserving the membrane integrity. Mechanistically, exposure of erythrocytes to pyocyanin showed increased cytosolic Ca(2+) activity as well as Ca(2+) -dependent proteolytic processing of μ-calpain. Pyocyanin further up-regulated erythrocyte ceramide abundance and triggered the production of reactive oxygen species. Pyocyanin-induced increased PS externalization in erythrocytes translated into enhanced prothrombin activation and fibrin generation in plasma. As judged by carboxyfluorescein succinimidyl-ester labelling, pyocyanin-treated erythrocytes were cleared faster from the murine circulation as compared to untreated erythrocytes. Furthermore, erythrocytes incubated in plasma from patients with P. aeruginosa sepsis showed increased PS exposure as compared to erythrocytes incubated in plasma from healthy donors. In conclusion, the present study discloses the eryptosis-inducing effect of the virulence factor pyocyanin, thereby shedding light on a potentially important mechanism in the systemic complications of P. aeruginosa infection." @default.
• W2301517287 created "2016-06-24" @default.
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• W2301517287 date "2016-01-19" @default.
• W2301517287 modified "2023-10-16" @default.
• W2301517287 title "Phosphatidylserine externalization and procoagulant activation of erythrocytes induced by <i>Pseudomonas aeruginosa</i> virulence factor pyocyanin" @default.
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• W2301517287 doi "https://doi.org/10.1111/jcmm.12778" @default.
• W2301517287 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5125577" @default.
• W2301517287 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/26781477" @default.
• W2301517287 hasPublicationYear "2016" @default.
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