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- W2301678655 abstract "Mechanosensitive ion channels are force-transducing enzymes that couple mechanical stimuli to ion flux. Understanding the gating mechanism of mechanosensitive channels is challenging because the stimulus seen by the channel reflects forces shared between the membrane, cytoskeleton and extracellular matrix. Here we examine whether the mechanosensitive channel PIEZO1 is activated by force-transmission through the bilayer. To achieve this, we generate HEK293 cell membrane blebs largely free of cytoskeleton. Using the bacterial channel MscL, we calibrate the bilayer tension demonstrating that activation of MscL in blebs is identical to that in reconstituted bilayers. Utilizing a novel PIEZO1-GFP fusion, we then show PIEZO1 is activated by bilayer tension in bleb membranes, gating at lower pressures indicative of removal of the cortical cytoskeleton and the mechanoprotection it provides. Thus, PIEZO1 channels must sense force directly transmitted through the bilayer." @default.
- W2301678655 created "2016-06-24" @default.
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- W2301678655 date "2016-01-20" @default.
- W2301678655 modified "2023-10-13" @default.
- W2301678655 title "Removal of the mechanoprotective influence of the cytoskeleton reveals PIEZO1 is gated by bilayer tension" @default.
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- W2301678655 doi "https://doi.org/10.1038/ncomms10366" @default.
- W2301678655 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4735864" @default.
- W2301678655 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/26785635" @default.
- W2301678655 hasPublicationYear "2016" @default.
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