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- W2301714202 abstract "Objective To study the antagonistic activities of Lactobacillus plantarum ST-Ⅲ against the adhesion of Escherichia coli and Salmonella enteritis to Caco-2 cells and explicate the phenomena of bacterial adhesion and inhibition.Method Fluorescence labeling with CFDA-SE was used to study the change of adhesive ability to Caco-2 cells of the two pathogens;the mechanisms of antagonistic activities were investigated by chemical and enzymatic treatments and extraction of component on the cell surface.Result ST-Ⅲ showed significant capability to inhibit the adhesion of E.coli and S.enteritis to Caco-2 cells.ST-Ⅲ had a better antagonistic effects against E.coli than against S.enteritis in the same situations.Chemical and enzymatic treatments showed the S-layer protein or lipoteichoic acid might be involved in the adhesion of ST-Ⅲ to Caco-2 cells.After extracted,the S-layer protein showed significantly inhibitory efforts to the adhesion of E.coli,but could not inhibit the adhesion of S.enteritis.The lipoteichoic acid on ST-Ⅲ cells did not affect the adhesion of pathogens in all experiments.Conclusion The antagonistic effect of ST-Ⅲ against the adhesion of E.coli on Caco-2 cells was ascribed to competitive inhibition,whereas the adhesion of S.enteritis to Caco-2 cells was mainly resulted from steric hindrance." @default.
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- W2301714202 date "2010-01-01" @default.
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- W2301714202 title "A fluorescence labeling method for the study on antagonistic activities of Lactobacillus plantarum ST-III against enteric pathogens and its related mechanism" @default.
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