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• W2301790301 abstract "Bacterial identification using rrs (16S rRNA) gene is widely reported. Bacteria possessing multiple copies of rrs lead to overestimation of its diversity. Staphylococcus genomes carries 5-6 copies of rrs showing high similarity in their nucleotide sequences, which lead to ambiguous results. The genomes of 31 strains of Staphylococcus representing 7 species were searched for the presence of common genes. In silico digestion of 34 common genes using 10 restriction endonucleases (REs) lead to select gene-RE combinations, which could be used as biomarkers. RE digestion of recA allowed unambiguous identification of 13 genomes representing all the 7 species. In addition, a few more genes (argH, argR, cysS, gyrB, purH, and pyrE) and RE combinations permitted further identification of 12 strains. By employing additional RE and genes unique to a particular strain, it was possible to identify the rest 6 Staphylococcus aureus strains. This approach has the potential to be utilized for rapid detection of Staphylococcus strains." @default.
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• W2301790301 date "2016-01-20" @default.
• W2301790301 modified "2023-09-25" @default.
• W2301790301 title "Searching Biomarkers in the Sequenced Genomes of Staphylococcus for their Rapid Identification" @default.
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• W2301790301 doi "https://doi.org/10.1007/s12088-016-0565-9" @default.
• W2301790301 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4729746" @default.
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