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- W2301845707 abstract "This protocol describes how to make semisynthetic gelatin methacryloyl (GelMA)-based hydrogels for use in 3D cell culture models for cancer research, stem cell research and tissue engineering. Progress in advancing a system-level understanding of the complexity of human tissue development and regeneration is hampered by a lack of biological model systems that recapitulate key aspects of these processes in a physiological context. Hence, growing demand by cell biologists for organ-specific extracellular mimics has led to the development of a plethora of 3D cell culture assays based on natural and synthetic matrices. We developed a physiological microenvironment of semisynthetic origin, called gelatin methacryloyl (GelMA)-based hydrogels, which combine the biocompatibility of natural matrices with the reproducibility, stability and modularity of synthetic biomaterials. We describe here a step-by-step protocol for the preparation of the GelMA polymer, which takes 1–2 weeks to complete, and which can be used to prepare hydrogel-based 3D cell culture models for cancer and stem cell research, as well as for tissue engineering applications. We also describe quality control and validation procedures, including how to assess the degree of GelMA functionalization and mechanical properties, to ensure reproducibility in experimental and animal studies." @default.
- W2301845707 created "2016-06-24" @default.
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- W2301845707 date "2016-03-17" @default.
- W2301845707 modified "2023-10-18" @default.
- W2301845707 title "Functionalization, preparation and use of cell-laden gelatin methacryloyl–based hydrogels as modular tissue culture platforms" @default.
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- W2301845707 doi "https://doi.org/10.1038/nprot.2016.037" @default.
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