FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2302463977> ?p ?o ?g. }

• W2302463977 abstract "Although thrombin has an important role in both central and peripheral nerve diseases, characterization of the anatomical distribution of its proteolytic activity has been limited by available methods. This study presents the development, challenges, validation and implementation of a novel histochemical method for visualization of thrombin activity in the nervous system. The method is based on the cleavage of the substrate, Boc-Asp(OBzl)-Pro-Arg-4MβNA by thrombin to liberate free 4-methoxy-2-naphthylamine (4MβNA). In the presence of 5-nitrosalicylaldehyde, free 4MβNA is captured, yielding an insoluble yellow fluorescent precipitate which marks the site of thrombin activity. The sensitivity of the method was determined in vitro using known concentrations of thrombin while the specificity was verified using a highly specific thrombin inhibitor. Using this method we determined the spatial distribution of thrombin activity in mouse brain following transient middle cerebral artery occlusion (tMCAo) and in mouse sciatic nerve following crush injury. Fluorescence microscopy revealed well-defined thrombin activity localized to the right ischemic hemisphere in cortical areas and in the striatum compared to negligible thrombin activity contralaterally. The histochemical localization of thrombin activity following tMCAo was in good correlation with the infarct areas per triphenyltetrazolium chloride staining and to thrombin activity measured biochemically in tissue punches (85 ± 35 and 20 ± 3 mU/ml, in the cortical and striatum areas respectively, compared to 7 ± 2 and 13 ± 2 mU/ml, in the corresponding contralateral areas; mean ± SEM; p<0.05). In addition, 24 h following crush injury, focal areas of highly elevated thrombin activity were detected in teased sciatic fibers. This observation was supported by the biochemical assay and western blot technique. The histochemical method developed in this study can serve as an important tool for studying the role of thrombin in physiological and pathological conditions." @default.
• W2302463977 created "2016-06-24" @default.
• W2302463977 creator A5006499979 @default.
• W2302463977 creator A5010664740 @default.
• W2302463977 creator A5024658913 @default.
• W2302463977 creator A5036766444 @default.
• W2302463977 creator A5053156520 @default.
• W2302463977 creator A5066754419 @default.
• W2302463977 creator A5072166912 @default.
• W2302463977 date "2016-04-01" @default.
• W2302463977 modified "2023-09-27" @default.
• W2302463977 title "A novel histochemical method for the visualization of thrombin activity in the nervous system" @default.
• W2302463977 cites W1493124133 @default.
• W2302463977 cites W1508836098 @default.
• W2302463977 cites W1546405566 @default.
• W2302463977 cites W1554082459 @default.
• W2302463977 cites W1555309153 @default.
• W2302463977 cites W1586665170 @default.
• W2302463977 cites W1594129908 @default.
• W2302463977 cites W1811402656 @default.
• W2302463977 cites W1920409153 @default.
• W2302463977 cites W1964296989 @default.
• W2302463977 cites W1973573196 @default.
• W2302463977 cites W1974659269 @default.
• W2302463977 cites W1974938721 @default.
• W2302463977 cites W1977186831 @default.
• W2302463977 cites W1979228901 @default.
• W2302463977 cites W1979790930 @default.
• W2302463977 cites W1983233834 @default.
• W2302463977 cites W1986962105 @default.
• W2302463977 cites W1989484029 @default.
• W2302463977 cites W1994591646 @default.
• W2302463977 cites W2010435582 @default.
• W2302463977 cites W2017554966 @default.
• W2302463977 cites W2017629587 @default.
• W2302463977 cites W2024028741 @default.
• W2302463977 cites W2027707602 @default.
• W2302463977 cites W2028341202 @default.
• W2302463977 cites W2032003104 @default.
• W2302463977 cites W2045079575 @default.
• W2302463977 cites W2053774332 @default.
• W2302463977 cites W2056303336 @default.
• W2302463977 cites W2056786932 @default.
• W2302463977 cites W2058798231 @default.
• W2302463977 cites W2072664770 @default.
• W2302463977 cites W2082077377 @default.
• W2302463977 cites W2085501100 @default.
• W2302463977 cites W2105503070 @default.
• W2302463977 cites W2105536474 @default.
• W2302463977 cites W2122415296 @default.
• W2302463977 cites W2122792200 @default.
• W2302463977 cites W2123140082 @default.
• W2302463977 cites W2130373760 @default.
• W2302463977 cites W2146721393 @default.
• W2302463977 cites W2153156911 @default.
• W2302463977 cites W2155198114 @default.
• W2302463977 cites W2160311718 @default.
• W2302463977 cites W2165201687 @default.
• W2302463977 cites W2171677112 @default.
• W2302463977 cites W2320708792 @default.
• W2302463977 cites W4299130242 @default.
• W2302463977 cites W2110016666 @default.
• W2302463977 doi "https://doi.org/10.1016/j.neuroscience.2016.01.065" @default.
• W2302463977 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/26851772" @default.
• W2302463977 hasPublicationYear "2016" @default.
• W2302463977 type Work @default.
• W2302463977 sameAs 2302463977 @default.
• W2302463977 citedByCount "9" @default.
• W2302463977 countsByYear W23024639772017 @default.
• W2302463977 countsByYear W23024639772018 @default.
• W2302463977 countsByYear W23024639772019 @default.
• W2302463977 countsByYear W23024639772020 @default.
• W2302463977 countsByYear W23024639772021 @default.
• W2302463977 countsByYear W23024639772022 @default.
• W2302463977 crossrefType "journal-article" @default.
• W2302463977 hasAuthorship W2302463977A5006499979 @default.
• W2302463977 hasAuthorship W2302463977A5010664740 @default.
• W2302463977 hasAuthorship W2302463977A5024658913 @default.
• W2302463977 hasAuthorship W2302463977A5036766444 @default.
• W2302463977 hasAuthorship W2302463977A5053156520 @default.
• W2302463977 hasAuthorship W2302463977A5066754419 @default.
• W2302463977 hasAuthorship W2302463977A5072166912 @default.
• W2302463977 hasConcept C105702510 @default.
• W2302463977 hasConcept C12554922 @default.
• W2302463977 hasConcept C126322002 @default.
• W2302463977 hasConcept C169760540 @default.
• W2302463977 hasConcept C185592680 @default.
• W2302463977 hasConcept C2777292125 @default.
• W2302463977 hasConcept C2780062018 @default.
• W2302463977 hasConcept C2781149210 @default.
• W2302463977 hasConcept C513476851 @default.
• W2302463977 hasConcept C529278444 @default.
• W2302463977 hasConcept C55493867 @default.
• W2302463977 hasConcept C71924100 @default.
• W2302463977 hasConcept C86803240 @default.
• W2302463977 hasConcept C89560881 @default.
• W2302463977 hasConceptScore W2302463977C105702510 @default.
• W2302463977 hasConceptScore W2302463977C12554922 @default.
• W2302463977 hasConceptScore W2302463977C126322002 @default.
• W2302463977 hasConceptScore W2302463977C169760540 @default.

• next