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- W2302783143 endingPage "1503" @default.
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- W2302783143 abstract "T lymphocytes stimulated through their antigen receptor (TCR) preferentially express mRNA isoforms with shorter 3´ untranslated regions (3´-UTRs) derived from alternative pre-mRNA cleavage and polyadenylation (APA). However, the physiological relevance of APA programs remains poorly understood. CD5 is a T-cell surface glycoprotein that negatively regulates TCR signaling from the onset of T-cell activation. CD5 plays a pivotal role in mediating outcomes of cell survival or apoptosis, and may prevent both autoimmunity and cancer. In human primary T lymphocytes and Jurkat cells we found three distinct mRNA isoforms encoding CD5, each derived from distinct poly(A) signals (PASs). Upon T-cell activation, there is an overall increase in CD5 mRNAs with a specific increase in the relative expression of the shorter isoforms. 3´-UTRs derived from these shorter isoforms confer higher reporter expression in activated T cells relative to the longer isoform. We further show that polypyrimidine tract binding protein (PTB/PTBP1) directly binds to the proximal PAS and PTB siRNA depletion causes a decrease in mRNA derived from this PAS, suggesting an effect on stability or poly(A) site selection to circumvent targeting of the longer CD5 mRNA isoform by miR-204. These mechanisms fine-tune CD5 expression levels and thus ultimately T-cell responses." @default.
- W2302783143 created "2016-06-24" @default.
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- W2302783143 date "2016-04-15" @default.
- W2302783143 modified "2023-10-12" @default.
- W2302783143 title "CD5 expression is regulated during human T-cell activation by alternative polyadenylation, PTBP1, and miR-204" @default.
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- W2302783143 doi "https://doi.org/10.1002/eji.201545663" @default.
- W2302783143 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5555168" @default.
- W2302783143 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/27005442" @default.
- W2302783143 hasPublicationYear "2016" @default.
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