Matches in SemOpenAlex for { <https://semopenalex.org/work/W2303836769> ?p ?o ?g. }
Showing items 1 to 85 of
85
with 100 items per page.
- W2303836769 abstract "Ras association domain family 1, isoform A (RASSF1A) is anovel tumor suppressor gene that is found to be inactivated inmore than 40 types of sporadic cancers. In addition, mouseRassf1a knockout models have an increased frequency ofspontaneous and induced tumors. The mechanisms by whichRASSF1A exerts its tumor suppression activities or thepathways it can regulate are not yet fully understood. Usingyeast two-hybrid system, we have previously identifiedC19ORF5/MAP1S as the major RASSF1A-interacting protein.C19ORF5 has two conserved microtubule-associated regionsand may function to anchor RASSF1A to the centrosomes.In this study, we have analyzed the cellular functions ofC19ORF5. By using small interfering RNA–mediated depletionand time-lapse video microscopy, we show that C19ORF5knockdown causes mitotic abnormalities that consist offailure to form a stable metaphase plate, premature sisterchromatid separation, lagging chromosomes, and multipolarspindles. We also show that a fraction of C19ORF5 localizes tothe spindle microtubules. Additionally, we show here thatC19ORF5 localizes to the microtubule-organizing centersduring microtubule regrowth after nocodazole washout.Knockdown of C19ORF5 disrupts the microtubule-organizingcenter and results in microtubule nucleation from severalsites. Whereas the localization of pericentrin is not affected,A- and ;-tubulin localization and sites of nucleation aregreatly altered by C19ORF5 depletion. This may indicatethat C19ORF5 plays a role in anchoring the microtubuleorganizingcenter to the centrosomes. In addition, we showthat the NH2 terminus of C19ORF5 is essential for this process.This novel role for C19ORF5 could explain the resultingmitotic abnormalities that occur on its depletion and canpotentially provide an underlying mechanism for the frequentcentrosome and microtubule abnormalities detected inseveral cancers." @default.
- W2303836769 created "2016-06-24" @default.
- W2303836769 creator A5064287491 @default.
- W2303836769 creator A5086175941 @default.
- W2303836769 date "2007-01-01" @default.
- W2303836769 modified "2023-09-23" @default.
- W2303836769 title "Depletion of the Ras Association Domain Family 1, Isoform A–Associated Novel Microtubule-Associated Protein, C19ORF5/MAP1S, Causes Mitotic Abnormalities" @default.
- W2303836769 hasPublicationYear "2007" @default.
- W2303836769 type Work @default.
- W2303836769 sameAs 2303836769 @default.
- W2303836769 citedByCount "0" @default.
- W2303836769 crossrefType "journal-article" @default.
- W2303836769 hasAuthorship W2303836769A5064287491 @default.
- W2303836769 hasAuthorship W2303836769A5086175941 @default.
- W2303836769 hasConcept C104317684 @default.
- W2303836769 hasConcept C10447061 @default.
- W2303836769 hasConcept C104672736 @default.
- W2303836769 hasConcept C142669718 @default.
- W2303836769 hasConcept C1491633281 @default.
- W2303836769 hasConcept C166703698 @default.
- W2303836769 hasConcept C173396325 @default.
- W2303836769 hasConcept C20418707 @default.
- W2303836769 hasConcept C2779363559 @default.
- W2303836769 hasConcept C29537977 @default.
- W2303836769 hasConcept C30481170 @default.
- W2303836769 hasConcept C47432576 @default.
- W2303836769 hasConcept C54355233 @default.
- W2303836769 hasConcept C67705224 @default.
- W2303836769 hasConcept C70278784 @default.
- W2303836769 hasConcept C70547292 @default.
- W2303836769 hasConcept C85813293 @default.
- W2303836769 hasConcept C86803240 @default.
- W2303836769 hasConcept C93304396 @default.
- W2303836769 hasConcept C94921385 @default.
- W2303836769 hasConcept C95444343 @default.
- W2303836769 hasConceptScore W2303836769C104317684 @default.
- W2303836769 hasConceptScore W2303836769C10447061 @default.
- W2303836769 hasConceptScore W2303836769C104672736 @default.
- W2303836769 hasConceptScore W2303836769C142669718 @default.
- W2303836769 hasConceptScore W2303836769C1491633281 @default.
- W2303836769 hasConceptScore W2303836769C166703698 @default.
- W2303836769 hasConceptScore W2303836769C173396325 @default.
- W2303836769 hasConceptScore W2303836769C20418707 @default.
- W2303836769 hasConceptScore W2303836769C2779363559 @default.
- W2303836769 hasConceptScore W2303836769C29537977 @default.
- W2303836769 hasConceptScore W2303836769C30481170 @default.
- W2303836769 hasConceptScore W2303836769C47432576 @default.
- W2303836769 hasConceptScore W2303836769C54355233 @default.
- W2303836769 hasConceptScore W2303836769C67705224 @default.
- W2303836769 hasConceptScore W2303836769C70278784 @default.
- W2303836769 hasConceptScore W2303836769C70547292 @default.
- W2303836769 hasConceptScore W2303836769C85813293 @default.
- W2303836769 hasConceptScore W2303836769C86803240 @default.
- W2303836769 hasConceptScore W2303836769C93304396 @default.
- W2303836769 hasConceptScore W2303836769C94921385 @default.
- W2303836769 hasConceptScore W2303836769C95444343 @default.
- W2303836769 hasIssue "2" @default.
- W2303836769 hasLocation W23038367691 @default.
- W2303836769 hasOpenAccess W2303836769 @default.
- W2303836769 hasPrimaryLocation W23038367691 @default.
- W2303836769 hasRelatedWork W2054036043 @default.
- W2303836769 hasRelatedWork W2066675073 @default.
- W2303836769 hasRelatedWork W2085411540 @default.
- W2303836769 hasRelatedWork W2091459179 @default.
- W2303836769 hasRelatedWork W2097749880 @default.
- W2303836769 hasRelatedWork W2099905734 @default.
- W2303836769 hasRelatedWork W2101475372 @default.
- W2303836769 hasRelatedWork W2107059185 @default.
- W2303836769 hasRelatedWork W2108888476 @default.
- W2303836769 hasRelatedWork W2126544765 @default.
- W2303836769 hasRelatedWork W2127183070 @default.
- W2303836769 hasRelatedWork W2133490488 @default.
- W2303836769 hasRelatedWork W2143925721 @default.
- W2303836769 hasRelatedWork W2268985380 @default.
- W2303836769 hasRelatedWork W2561261294 @default.
- W2303836769 hasRelatedWork W2561895286 @default.
- W2303836769 hasRelatedWork W2912395639 @default.
- W2303836769 hasRelatedWork W3173704743 @default.
- W2303836769 hasRelatedWork W790913356 @default.
- W2303836769 hasRelatedWork W2513403388 @default.
- W2303836769 hasVolume "67" @default.
- W2303836769 isParatext "false" @default.
- W2303836769 isRetracted "false" @default.
- W2303836769 magId "2303836769" @default.
- W2303836769 workType "article" @default.