Matches in SemOpenAlex for { <https://semopenalex.org/work/W2305397730> ?p ?o ?g. }
- W2305397730 endingPage "563" @default.
- W2305397730 startingPage "557" @default.
- W2305397730 abstract "Amino terminal Cu(II)- and Ni(II)-binding (ATCUN) motif is included in a protein or peptide with a structural characterization comprising of a free NH2-terminus, a histidine residue in the third position and two intervening peptide nitrogens. We reported a signal-on electrochemical strategy for detection of protease based on the formation of ATCUN-Cu(II) on electrode surface. In the method, an amino residue in the native peptide substrate was replaced with a histidine. Cleavage of the sequence-specific peptide pre-immobilized on an electrode by the protease led to the presence of ATCUN motif and the formation of ATCUN-Cu(II) in the presence of Cu(II). Consequently, a voltammetric signal was observed. Contrarily, inhibition of protease by its potential inhibitor decreased the voltammetric response. To demonstrate the feasibility and sensitivity of our strategy, β-secretase was tested as a model protease. As a result, a detection limit of 0.08 nmol L−1 was achieved. The half-maximum inhibition value (IC50) of a well-known β-secretase inhibitor was found to be 22.6 nmol L−1, which is in agreement with the reported value." @default.
- W2305397730 created "2016-06-24" @default.
- W2305397730 creator A5035648686 @default.
- W2305397730 creator A5040647242 @default.
- W2305397730 creator A5046871461 @default.
- W2305397730 creator A5064084098 @default.
- W2305397730 creator A5064474253 @default.
- W2305397730 creator A5071811404 @default.
- W2305397730 creator A5014399759 @default.
- W2305397730 date "2016-09-01" @default.
- W2305397730 modified "2023-10-09" @default.
- W2305397730 title "A signal-on electrochemical strategy for protease detection based on the formation of ATCUN-Cu(II)" @default.
- W2305397730 cites W1534001036 @default.
- W2305397730 cites W1831306918 @default.
- W2305397730 cites W1854114594 @default.
- W2305397730 cites W1967038821 @default.
- W2305397730 cites W1972812195 @default.
- W2305397730 cites W1978797679 @default.
- W2305397730 cites W1981685208 @default.
- W2305397730 cites W1984013219 @default.
- W2305397730 cites W1989003785 @default.
- W2305397730 cites W1991889711 @default.
- W2305397730 cites W1996854829 @default.
- W2305397730 cites W2000539928 @default.
- W2305397730 cites W2001526873 @default.
- W2305397730 cites W2013234744 @default.
- W2305397730 cites W2015709915 @default.
- W2305397730 cites W2030641075 @default.
- W2305397730 cites W2037835521 @default.
- W2305397730 cites W2038286728 @default.
- W2305397730 cites W2041514350 @default.
- W2305397730 cites W2042106585 @default.
- W2305397730 cites W2047245081 @default.
- W2305397730 cites W2050445499 @default.
- W2305397730 cites W2051057705 @default.
- W2305397730 cites W2053103992 @default.
- W2305397730 cites W2054831936 @default.
- W2305397730 cites W2055294137 @default.
- W2305397730 cites W2059495630 @default.
- W2305397730 cites W2061721435 @default.
- W2305397730 cites W2066873026 @default.
- W2305397730 cites W2073909430 @default.
- W2305397730 cites W2076658253 @default.
- W2305397730 cites W2080141125 @default.
- W2305397730 cites W2080978123 @default.
- W2305397730 cites W208110057 @default.
- W2305397730 cites W2083185738 @default.
- W2305397730 cites W2095421113 @default.
- W2305397730 cites W2114872349 @default.
- W2305397730 cites W2122926059 @default.
- W2305397730 cites W2123166384 @default.
- W2305397730 cites W2145549507 @default.
- W2305397730 cites W2155300042 @default.
- W2305397730 cites W2163523761 @default.
- W2305397730 cites W2173165970 @default.
- W2305397730 cites W2314190978 @default.
- W2305397730 cites W2318179308 @default.
- W2305397730 cites W2321241112 @default.
- W2305397730 cites W2417753269 @default.
- W2305397730 cites W4210955468 @default.
- W2305397730 cites W427749411 @default.
- W2305397730 doi "https://doi.org/10.1016/j.snb.2016.03.144" @default.
- W2305397730 hasPublicationYear "2016" @default.
- W2305397730 type Work @default.
- W2305397730 sameAs 2305397730 @default.
- W2305397730 citedByCount "18" @default.
- W2305397730 countsByYear W23053977302016 @default.
- W2305397730 countsByYear W23053977302017 @default.
- W2305397730 countsByYear W23053977302018 @default.
- W2305397730 countsByYear W23053977302019 @default.
- W2305397730 countsByYear W23053977302021 @default.
- W2305397730 countsByYear W23053977302022 @default.
- W2305397730 countsByYear W23053977302023 @default.
- W2305397730 crossrefType "journal-article" @default.
- W2305397730 hasAuthorship W2305397730A5014399759 @default.
- W2305397730 hasAuthorship W2305397730A5035648686 @default.
- W2305397730 hasAuthorship W2305397730A5040647242 @default.
- W2305397730 hasAuthorship W2305397730A5046871461 @default.
- W2305397730 hasAuthorship W2305397730A5064084098 @default.
- W2305397730 hasAuthorship W2305397730A5064474253 @default.
- W2305397730 hasAuthorship W2305397730A5071811404 @default.
- W2305397730 hasConcept C119128265 @default.
- W2305397730 hasConcept C147789679 @default.
- W2305397730 hasConcept C17525397 @default.
- W2305397730 hasConcept C181199279 @default.
- W2305397730 hasConcept C185592680 @default.
- W2305397730 hasConcept C21951064 @default.
- W2305397730 hasConcept C2776714187 @default.
- W2305397730 hasConcept C2778460671 @default.
- W2305397730 hasConcept C2779281246 @default.
- W2305397730 hasConcept C2781338088 @default.
- W2305397730 hasConcept C43617362 @default.
- W2305397730 hasConcept C52859227 @default.
- W2305397730 hasConcept C55493867 @default.
- W2305397730 hasConcept C71240020 @default.
- W2305397730 hasConceptScore W2305397730C119128265 @default.
- W2305397730 hasConceptScore W2305397730C147789679 @default.
- W2305397730 hasConceptScore W2305397730C17525397 @default.