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- W2305613034 abstract "Objective To breed 5-methyltetrahydrofolate-producing(5-MTHF-producing) double resistance mutant and investigate the contribution of fermentation time and temperature to accumulation forms of folate.Methods The original strain was induced by UV and EMS,then checked out by sulfanilamide or methotrexate medium.The influence of fermentation conditions on accumulation forms of folate was tested by single factor analysis.The content and existent form of folate were tested by HPLC.Results The double resistance mutant with stable folate production was obtained and the output of folate was 2.2-fold than that of the original strain.The most suitable fermentation time for the accumulation of 5-MTHF was 16 hour and the yield of accumulation was 68.94 μg/g yeast.Conclusion The yield of folate can be improved for the resistance mutant.The temperature has no notable contribution to the forms and yield of folate,whereas the content of 5-MTHF can be highly dependent on the fermentation time." @default.
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- W2305613034 date "2010-01-01" @default.
- W2305613034 modified "2023-09-24" @default.
- W2305613034 title "Breeding of 5-methyltetrahydrofolate-producing double resistance mutant and optimization of accumulation condition." @default.
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