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- W2305688642 abstract "Objective To study the effects of the spent culture supernatant(SCS) of Lactobacillus acidophilus strain LA14 on the intestinal movement in mice.Method 50 mice were randomly divided into 5 groups.SCS,liquor of living bacteria,SCS plus liquor of living bacteria [25 ml/(kg·times)],atropine sulfate injection [10 mg/(kg·times)] or the same volume of normal saline were respectively administered(two times per day,ig)to mice for three days.After one hour of the last administration,all mice were administered Indian ink 0.2 ml/per body,ig.20 minutes later,the mice were executed.The whole length of small intestine and the distance that the Indian ink was propelled were measured.The ink propellant rate and the inhibitory rate were calculated.Otherwise,60 mice were randomly divided into 6 groups.SCS,liquor of living bacteria,SCS plus liquor of living bacteria [25 ml/(kg·times)],Smectite [1.5 g/(kg·times)] or the same volume of normal saline were respectively administered(two times per day,ig) to mice for three days.After one hour of the last administration,the mice except the group of normal saline were administered neostigmine methylsulfate injection 2 mg/kg,ig.10 minutes later,all mice were administered Indian ink 0.2 ml/per body,ig.10 minites later,the mice were executed.The measure and calculation were done as above.Result In SCS group,SCS plus living bacteria group and atropine group,the ink propellant rate in normal mice was remarkably reduced.Compared with normal saline group,the difference was significant(P0.01).In the model group induced by oral administration of neostigmine,the small intestinal peristalsis in mice was enhanced and the ink propellant rate was obviously raised.Compared with normal saline group,the difference was significant(P0.01).After administering SCS,living bacteria,SCS plus living bacteria and Smectite,the hyperfunction of small intestinal peristalsis induced by neostigmine was markedly inhibited and the ink propellant rate was largely reduced.Compared with model group,the difference was statistically significant(P0.01).Conclusion SCS of Lactobacillus acidophilus has an obvious effect on inhibiting the propellant movement of small intestine in normal mice.SCS and living bacteria can significant inhibit the hyperfunction of small intestinal movement induced by neostigmine in mice." @default.
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- W2305688642 date "2010-01-01" @default.
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- W2305688642 title "Effects of spent culture supernatant of Lactobacillus acidophilus on the intestinal movement in mice." @default.
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