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- W2306723710 abstract "// Xianyu Li 1,2 , Jing Jiang 1 , Xinyuan Zhao 1 , Yan Zhao 1 , Qichen Cao 1 , Qing Zhao 1 , Huanhuan Han 1 , Jifeng Wang 1 , Zixiang Yu 1 , Bo Peng 1 , Wantao Ying 1 and Xiaohong Qian 1 1 National Center for Protein Sciences Beijing, State Key Laboratory of Proteomics, Beijing Proteome Research Center, Beijing Institute of Radiation Medicine, Beijing, China 2 Beijing Key Laboratory of Traditional Chinese Medicine Basic Research on Prevention and Treatment for Major Diseases, Experimental Research Center, China Academy of Chinese Medical Sciences, Beijing, China Correspondence to: Wantao Ying, email: // Xiaohong Qian, email: // Keywords : hepatocellular carcinoma, metastasis, secretome, OFFGEL fractionation, zic-HILIC, label-free quantitation, N-glycoproteomics Received : July 26, 2015 Accepted : February 05, 2016 Published : March 21, 2016 Abstract Cancer cell metastasis is a major cause of cancer fatality. But the underlying molecular mechanisms remain incompletely understood, which results in the lack of efficient diagnosis, therapy and prevention approaches. Here, we report a systematic study on the secretory proteins (secretome) and secretory N-glycoproteins (N-glycosecretome) of four human hepatocellular carcinoma (HCC) cell lines with different metastatic potential, to explore the molecular mechanism of metastasis and supply the clues for effective measurement of diagnosis and therapy. Totally, 6242 unique gene products (GPs) and 1637 unique N-glycosites from 635 GPs were confidently identified. About 4000 GPs on average were quantified in each of the cell lines, 1156 of which show differential expression (p<0.05). Ninety-nine percentage of the significantly altered proteins were secretory proteins and proteins correlated to cell movement were significantly activated with the increasing of metastatic potential of the cell lines. Twenty-three GPs increased both in the secretome and the N-glycosecretome were chosen as candidates and verified by western blot analysis, and 10 of them were chosen for immunohistochemistry (IHC) analysis. The cumulative survival rates of the patients with candidate (FAT1, DKK3) suggested that these proteins might be used as biomarkers for HCC diagnosis. In addition, a comparative analysis with the published core human plasma database (1754 GPs) revealed that there were 182 proteins not presented in the human plasma database but identified by our studies, some of which were selected and verified successfully by western blotting in human plasma." @default.
- W2306723710 created "2016-06-24" @default.
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- W2306723710 date "2016-03-21" @default.
- W2306723710 modified "2023-09-23" @default.
- W2306723710 title "In-depth analysis of secretome and N-glycosecretome of human hepatocellular carcinoma metastatic cell lines shed light on metastasis correlated proteins" @default.
- W2306723710 cites W1516095885 @default.
- W2306723710 cites W1953681441 @default.
- W2306723710 cites W1963977752 @default.
- W2306723710 cites W1965917470 @default.
- W2306723710 cites W1969836997 @default.
- W2306723710 cites W1972468747 @default.
- W2306723710 cites W1975290591 @default.
- W2306723710 cites W1976396776 @default.
- W2306723710 cites W1977692564 @default.
- W2306723710 cites W1979803040 @default.
- W2306723710 cites W1981294952 @default.
- W2306723710 cites W1981989535 @default.
- W2306723710 cites W1994927207 @default.
- W2306723710 cites W1995249913 @default.
- W2306723710 cites W1998536894 @default.
- W2306723710 cites W2000973093 @default.
- W2306723710 cites W2002790336 @default.
- W2306723710 cites W2004579735 @default.
- W2306723710 cites W2007601283 @default.
- W2306723710 cites W2014875090 @default.
- W2306723710 cites W2015981241 @default.
- W2306723710 cites W2017280655 @default.
- W2306723710 cites W2023046869 @default.
- W2306723710 cites W2024191848 @default.
- W2306723710 cites W2024926441 @default.
- W2306723710 cites W2028554313 @default.
- W2306723710 cites W2031079181 @default.
- W2306723710 cites W2031704362 @default.
- W2306723710 cites W2032600439 @default.
- W2306723710 cites W2035003769 @default.
- W2306723710 cites W2037199116 @default.
- W2306723710 cites W2039142185 @default.
- W2306723710 cites W2040756457 @default.
- W2306723710 cites W2058430410 @default.
- W2306723710 cites W2077065650 @default.
- W2306723710 cites W2077687291 @default.
- W2306723710 cites W2080257751 @default.
- W2306723710 cites W2082693597 @default.
- W2306723710 cites W2086454839 @default.
- W2306723710 cites W2088618556 @default.
- W2306723710 cites W2092853779 @default.
- W2306723710 cites W2093988614 @default.
- W2306723710 cites W2096224160 @default.
- W2306723710 cites W2096642770 @default.
- W2306723710 cites W2101614917 @default.
- W2306723710 cites W2103017472 @default.
- W2306723710 cites W2104325455 @default.
- W2306723710 cites W2106842540 @default.
- W2306723710 cites W2111086397 @default.
- W2306723710 cites W2114063504 @default.
- W2306723710 cites W2117692326 @default.
- W2306723710 cites W2117839432 @default.
- W2306723710 cites W2123242217 @default.
- W2306723710 cites W2125582000 @default.
- W2306723710 cites W2134494394 @default.
- W2306723710 cites W2140442587 @default.
- W2306723710 cites W2141119715 @default.
- W2306723710 cites W2142286883 @default.
- W2306723710 cites W2143026523 @default.
- W2306723710 cites W2143534453 @default.
- W2306723710 cites W2148241996 @default.
- W2306723710 cites W2149545014 @default.
- W2306723710 cites W2153243072 @default.
- W2306723710 cites W2154279182 @default.
- W2306723710 cites W2158217645 @default.
- W2306723710 cites W2158274171 @default.
- W2306723710 cites W2159698797 @default.
- W2306723710 cites W2161098464 @default.
- W2306723710 cites W2172116519 @default.
- W2306723710 cites W2321423077 @default.
- W2306723710 cites W326741768 @default.
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- W2306723710 doi "https://doi.org/10.18632/oncotarget.8247" @default.
- W2306723710 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5008342" @default.
- W2306723710 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/27014972" @default.
- W2306723710 hasPublicationYear "2016" @default.
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