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- W2307313718 abstract "DNA templates containing a set of base modifications in the major groove (5-substituted pyrimidines or 7-substituted 7-deazapurines bearing H, methyl, vinyl, ethynyl or phenyl groups) were prepared by PCR using the corresponding base-modified 2′-deoxyribonucleoside triphosphates (dNTPs). The modified templates were used in an in vitro transcription assay using RNA polymerase from Bacillus subtilis and Escherichia coli. Some modified nucleobases bearing smaller modifications (H, Me in 7-deazapurines) were perfectly tolerated by both enzymes, whereas bulky modifications (Ph at any nucleobase) and, surprisingly, uracil blocked transcription. Some middle-sized modifications (vinyl or ethynyl) were partly tolerated mostly by the E. coli enzyme. In all cases where the transcription proceeded, full length RNA product with correct sequence was obtained indicating that the modifications of the template are not mutagenic and the inhibition is probably at the stage of initiation. The results are promising for the development of bioorthogonal reactions for artificial chemical switching of the transcription." @default.
- W2307313718 created "2016-06-24" @default.
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- W2307313718 date "2016-03-21" @default.
- W2307313718 modified "2023-10-18" @default.
- W2307313718 title "Influence of major-groove chemical modifications of DNA on transcription by bacterial RNA polymerases" @default.
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- W2307313718 doi "https://doi.org/10.1093/nar/gkw171" @default.
- W2307313718 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4838386" @default.
- W2307313718 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/27001521" @default.
- W2307313718 hasPublicationYear "2016" @default.
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