Matches in SemOpenAlex for { <https://semopenalex.org/work/W2307341708> ?p ?o ?g. }
Showing items 1 to 84 of
84
with 100 items per page.
- W2307341708 endingPage "418" @default.
- W2307341708 startingPage "409" @default.
- W2307341708 abstract "AbstractImmunoblotting provides a simple and effective method for identifying specific antigens in a complex mixture of proteins. Initially, the constituent polypeptides are separated using SDS-polyacrylamide gel electrophoresis, or a similar technique, and then are transferred either electrophoretically or by diffusion onto a nitrocellulose filter. The transfer of proteins from gels onto membranes is discussed in vol. 1 of this series and Chapters 28 and 29, this volume. Once immobilized on a sheet of nitrocellulose, specific polypeptides can be identified using antibodies that bind to antigens retained on the filter. In this chapter we describe conditions appropriate for binding antibodies to immobilized proteins and a method for locating these antibody-antigen complexes using 125I-labeled protein A. These methods are based on those of Burnett (1) and Towbin et al. (2).KeywordsNitrocellulose FilterSpecific Antibody TiterAmido BlackSpecific PolypeptidePure AntigenThese keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves." @default.
- W2307341708 created "2016-06-24" @default.
- W2307341708 creator A5026723191 @default.
- W2307341708 creator A5064104793 @default.
- W2307341708 date "2003-11-14" @default.
- W2307341708 modified "2023-09-24" @default.
- W2307341708 title "Immunodetection of Proteins on Western Blots Using <SUP>125</SUP>I-Labeled Protein A" @default.
- W2307341708 cites W2061792951 @default.
- W2307341708 doi "https://doi.org/10.1385/0-89603-126-8:409" @default.
- W2307341708 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21400180" @default.
- W2307341708 hasPublicationYear "2003" @default.
- W2307341708 type Work @default.
- W2307341708 sameAs 2307341708 @default.
- W2307341708 citedByCount "6" @default.
- W2307341708 countsByYear W23073417082013 @default.
- W2307341708 countsByYear W23073417082014 @default.
- W2307341708 countsByYear W23073417082015 @default.
- W2307341708 crossrefType "book-chapter" @default.
- W2307341708 hasAuthorship W2307341708A5026723191 @default.
- W2307341708 hasAuthorship W2307341708A5064104793 @default.
- W2307341708 hasConcept C101660505 @default.
- W2307341708 hasConcept C104317684 @default.
- W2307341708 hasConcept C116084860 @default.
- W2307341708 hasConcept C144647389 @default.
- W2307341708 hasConcept C147483822 @default.
- W2307341708 hasConcept C153911025 @default.
- W2307341708 hasConcept C159654299 @default.
- W2307341708 hasConcept C181199279 @default.
- W2307341708 hasConcept C185592680 @default.
- W2307341708 hasConcept C188027245 @default.
- W2307341708 hasConcept C203014093 @default.
- W2307341708 hasConcept C2778862431 @default.
- W2307341708 hasConcept C2778954975 @default.
- W2307341708 hasConcept C2994508507 @default.
- W2307341708 hasConcept C40250595 @default.
- W2307341708 hasConcept C41625074 @default.
- W2307341708 hasConcept C43617362 @default.
- W2307341708 hasConcept C54355233 @default.
- W2307341708 hasConcept C55493867 @default.
- W2307341708 hasConcept C7051213 @default.
- W2307341708 hasConcept C85265743 @default.
- W2307341708 hasConcept C86803240 @default.
- W2307341708 hasConceptScore W2307341708C101660505 @default.
- W2307341708 hasConceptScore W2307341708C104317684 @default.
- W2307341708 hasConceptScore W2307341708C116084860 @default.
- W2307341708 hasConceptScore W2307341708C144647389 @default.
- W2307341708 hasConceptScore W2307341708C147483822 @default.
- W2307341708 hasConceptScore W2307341708C153911025 @default.
- W2307341708 hasConceptScore W2307341708C159654299 @default.
- W2307341708 hasConceptScore W2307341708C181199279 @default.
- W2307341708 hasConceptScore W2307341708C185592680 @default.
- W2307341708 hasConceptScore W2307341708C188027245 @default.
- W2307341708 hasConceptScore W2307341708C203014093 @default.
- W2307341708 hasConceptScore W2307341708C2778862431 @default.
- W2307341708 hasConceptScore W2307341708C2778954975 @default.
- W2307341708 hasConceptScore W2307341708C2994508507 @default.
- W2307341708 hasConceptScore W2307341708C40250595 @default.
- W2307341708 hasConceptScore W2307341708C41625074 @default.
- W2307341708 hasConceptScore W2307341708C43617362 @default.
- W2307341708 hasConceptScore W2307341708C54355233 @default.
- W2307341708 hasConceptScore W2307341708C55493867 @default.
- W2307341708 hasConceptScore W2307341708C7051213 @default.
- W2307341708 hasConceptScore W2307341708C85265743 @default.
- W2307341708 hasConceptScore W2307341708C86803240 @default.
- W2307341708 hasLocation W23073417081 @default.
- W2307341708 hasLocation W23073417082 @default.
- W2307341708 hasOpenAccess W2307341708 @default.
- W2307341708 hasPrimaryLocation W23073417081 @default.
- W2307341708 hasRelatedWork W156659529 @default.
- W2307341708 hasRelatedWork W1922850604 @default.
- W2307341708 hasRelatedWork W1969601362 @default.
- W2307341708 hasRelatedWork W2030366638 @default.
- W2307341708 hasRelatedWork W2064695980 @default.
- W2307341708 hasRelatedWork W2080343926 @default.
- W2307341708 hasRelatedWork W2086487735 @default.
- W2307341708 hasRelatedWork W2111817213 @default.
- W2307341708 hasRelatedWork W2399234920 @default.
- W2307341708 hasRelatedWork W4243376080 @default.
- W2307341708 isParatext "false" @default.
- W2307341708 isRetracted "false" @default.
- W2307341708 magId "2307341708" @default.
- W2307341708 workType "book-chapter" @default.