Matches in SemOpenAlex for { <https://semopenalex.org/work/W2308205458> ?p ?o ?g. }
Showing items 1 to 83 of
83
with 100 items per page.
- W2308205458 abstract "DNA cages are polyhedra self-assembled from synthetic oligonucleotides in a one-pot process. The main system described in this thesis is a reconfigurable, wire-framed DNA tetrahedron in nanometre-scale. On one of its vertices this tetrahedron has an overhang that can hybridise with a specific sequence of nucleic acids and open the cage. We describe the design of a reconfigurable cage that remained closed under physiological conditions and only opened in the presence of an appropriate signal in solution. Fluorescence techniques were employed to distinguish the open and closed states of the cage. We used flow cytometry and confocal microscopy to successfully established the open and closed states of the cage inside live cultured mammalian cells. Further experiments revealed that the DNA cage could be opened by a separately transfected signalling strand. Hybridisation between two separately transfected systems was possible. The DNA cage was then simplified to a DNA duplex so that the intracellular interactions between the two nucleic acids systems could be studied more efficiently. Microscopy images showed that the interaction occurred in membrane-bound compartments. We describe an investigation into the use of various cellular RNAs, including full-length mRNA and tRNA-RNA fusion, to actuate the DNA cages. Choosing an appropriate cellular opening signal remains a challenge. Analysis showed that bulky cellular RNA experienced steric hindrance with the rigid DNA cage. Finally, other potential biological applications of DNA cages, such as using DNA nanostructures as the carriers for genetic therapeutic agents, were also presented." @default.
- W2308205458 created "2016-06-24" @default.
- W2308205458 creator A5088533742 @default.
- W2308205458 date "2015-01-01" @default.
- W2308205458 modified "2023-09-27" @default.
- W2308205458 title "Actuation of DNA cages and their potential biological applications" @default.
- W2308205458 hasPublicationYear "2015" @default.
- W2308205458 type Work @default.
- W2308205458 sameAs 2308205458 @default.
- W2308205458 citedByCount "0" @default.
- W2308205458 crossrefType "dissertation" @default.
- W2308205458 hasAuthorship W2308205458A5088533742 @default.
- W2308205458 hasConcept C104317684 @default.
- W2308205458 hasConcept C114614502 @default.
- W2308205458 hasConcept C118630614 @default.
- W2308205458 hasConcept C121332964 @default.
- W2308205458 hasConcept C12554922 @default.
- W2308205458 hasConcept C129275984 @default.
- W2308205458 hasConcept C129312508 @default.
- W2308205458 hasConcept C160272327 @default.
- W2308205458 hasConcept C169274487 @default.
- W2308205458 hasConcept C171250308 @default.
- W2308205458 hasConcept C185592680 @default.
- W2308205458 hasConcept C191961535 @default.
- W2308205458 hasConcept C192562407 @default.
- W2308205458 hasConcept C24107716 @default.
- W2308205458 hasConcept C33923547 @default.
- W2308205458 hasConcept C54009773 @default.
- W2308205458 hasConcept C552990157 @default.
- W2308205458 hasConcept C55493867 @default.
- W2308205458 hasConcept C62520636 @default.
- W2308205458 hasConcept C67705224 @default.
- W2308205458 hasConcept C86803240 @default.
- W2308205458 hasConcept C91881484 @default.
- W2308205458 hasConceptScore W2308205458C104317684 @default.
- W2308205458 hasConceptScore W2308205458C114614502 @default.
- W2308205458 hasConceptScore W2308205458C118630614 @default.
- W2308205458 hasConceptScore W2308205458C121332964 @default.
- W2308205458 hasConceptScore W2308205458C12554922 @default.
- W2308205458 hasConceptScore W2308205458C129275984 @default.
- W2308205458 hasConceptScore W2308205458C129312508 @default.
- W2308205458 hasConceptScore W2308205458C160272327 @default.
- W2308205458 hasConceptScore W2308205458C169274487 @default.
- W2308205458 hasConceptScore W2308205458C171250308 @default.
- W2308205458 hasConceptScore W2308205458C185592680 @default.
- W2308205458 hasConceptScore W2308205458C191961535 @default.
- W2308205458 hasConceptScore W2308205458C192562407 @default.
- W2308205458 hasConceptScore W2308205458C24107716 @default.
- W2308205458 hasConceptScore W2308205458C33923547 @default.
- W2308205458 hasConceptScore W2308205458C54009773 @default.
- W2308205458 hasConceptScore W2308205458C552990157 @default.
- W2308205458 hasConceptScore W2308205458C55493867 @default.
- W2308205458 hasConceptScore W2308205458C62520636 @default.
- W2308205458 hasConceptScore W2308205458C67705224 @default.
- W2308205458 hasConceptScore W2308205458C86803240 @default.
- W2308205458 hasConceptScore W2308205458C91881484 @default.
- W2308205458 hasLocation W23082054581 @default.
- W2308205458 hasOpenAccess W2308205458 @default.
- W2308205458 hasPrimaryLocation W23082054581 @default.
- W2308205458 hasRelatedWork W1527205823 @default.
- W2308205458 hasRelatedWork W1978421637 @default.
- W2308205458 hasRelatedWork W1993898634 @default.
- W2308205458 hasRelatedWork W1999129518 @default.
- W2308205458 hasRelatedWork W2058329064 @default.
- W2308205458 hasRelatedWork W2087282559 @default.
- W2308205458 hasRelatedWork W2089777255 @default.
- W2308205458 hasRelatedWork W2147754270 @default.
- W2308205458 hasRelatedWork W2154224094 @default.
- W2308205458 hasRelatedWork W2156681855 @default.
- W2308205458 hasRelatedWork W2326528202 @default.
- W2308205458 hasRelatedWork W2397271466 @default.
- W2308205458 hasRelatedWork W2560481334 @default.
- W2308205458 hasRelatedWork W2780466662 @default.
- W2308205458 hasRelatedWork W2809884438 @default.
- W2308205458 hasRelatedWork W2905854260 @default.
- W2308205458 hasRelatedWork W2908370653 @default.
- W2308205458 hasRelatedWork W2987147669 @default.
- W2308205458 hasRelatedWork W3172942648 @default.
- W2308205458 hasRelatedWork W3214683560 @default.
- W2308205458 isParatext "false" @default.
- W2308205458 isRetracted "false" @default.
- W2308205458 magId "2308205458" @default.
- W2308205458 workType "dissertation" @default.