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- W2308485616 abstract "Isolated cardiac tissue allows investigators to study mechanisms underlying normal and pathological conditions, which would otherwise be difficult or impossible to perform in vivo. Cultured neonatal rat ventricular cardiac myocytes (NRVM) are widely used to study signaling and growth mechanisms in the heart, primarily due to the versatility, economy, and convenience of this in vitro model. However, the lack of a well‐defined longitudinal cellular axis greatly hampers the ability to measure contractile function in these cells, and therefore to associate signaling with mechanical function. In these methods, we demonstrate that this limitation can be overcome by using papillary muscles isolated from neonatal rat hearts. In the methods we describe procedures for isolation of right ventricular papillary muscles from 3‐day‐old neonatal rats and effects of mechanical and humoral stimuli on contraction and relaxation properties of these tissues." @default.
- W2308485616 created "2016-06-24" @default.
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- W2308485616 date "2016-02-01" @default.
- W2308485616 modified "2023-10-17" @default.
- W2308485616 title "Isolated neonatal rat papillary muscles: a new model to translate neonatal rat myocyte signaling into contractile mechanics" @default.
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- W2308485616 doi "https://doi.org/10.14814/phy2.12694" @default.
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