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- W2308529903 abstract "Anaerobic conditions are necessary to prevent the autoxidation of tetrahydrobiopterin. An enzymatic method for achieving such an anaerobic condition is shown to be obtained by a lactate oxidase activity in the membrane of Escherichia coli when the enzyme concentration exceeds 0.05 unit/ml. A procedure is described for partial purification of the membrane fragment that involves salt precipitation and gel sieve chromatography on Bio-Gel A-50m. The rate of removal of oxygen is used to define the enzyme activity, to determine stability during storage, and to define conditions for stabilization of tetrahydrobiopterin. A second assay procedure that measures the rate of reduction of resazurin is described and is useful when the enzyme concentration is low." @default.
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- W2308529903 date "1987-10-01" @default.
- W2308529903 modified "2023-09-26" @default.
- W2308529903 title "Partial purification of an oxygen scavenging cell membrane fraction for use in anaerobic biochemical reactions." @default.
- W2308529903 doi "https://doi.org/10.1111/j.1470-8744.1987.tb00485.x" @default.
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