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- W2308581254 endingPage "1126" @default.
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- W2308581254 abstract "Signaling proteins comprise interaction and effector modules connected by linkers. Throughout evolution, these recurring modules have multiply been recombined to produce the present-day plethora of signaling proteins. Likewise, modular recombination lends itself to the engineering of hybrid signal receptors, whose functionality hinges on linker topology, sequence, and length. Often, numerous linkers must be assessed to obtain functional receptors. To expedite linker optimization, we devised the PATCHY strategy (primer-aided truncation for the creation of hybrid proteins) for the facile construction of hybrid gene libraries with defined linker distributions. Empowered by PATCHY, we engineered photoreceptors whose signal response was governed by linker length: whereas blue-light-repressed variants possessed linkers of 7n or 7n+5 residues, variants with 7n+1 residues were blue-light-activated. Related natural receptors predominantly displayed linker lengths of 7n and 7n+5 residues but rarely of 7n+1 residues. PATCHY efficiently explores linker sequence space to yield functional hybrid proteins including variants transcending the natural repertoire of signaling proteins." @default.
- W2308581254 created "2016-06-24" @default.
- W2308581254 creator A5010953596 @default.
- W2308581254 creator A5019372461 @default.
- W2308581254 creator A5058235831 @default.
- W2308581254 creator A5088264372 @default.
- W2308581254 date "2016-03-29" @default.
- W2308581254 modified "2023-09-30" @default.
- W2308581254 title "Library-Aided Probing of Linker Determinants in Hybrid Photoreceptors" @default.
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- W2308581254 doi "https://doi.org/10.1021/acssynbio.6b00028" @default.
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- W2308581254 hasPublicationYear "2016" @default.
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