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- W2308985385 abstract "The conditions of extraction and quantification of activity during interaction of carnation and Fusarium oxysporum f. sp. dianthi race 2 were selected to evaluate the dynamics of the enzyme Phenylalanine Ammonium Lyase (PAL) at the carnations steam. For the extraction from roots and stems a previous treatment with acetone and extraction with borate pH 8.8, EDTA 2 mM and mercaptoethanol 18 mM buffer were selected. For the quantification at steam level a discontinous assay for 10 min at 37 °C, pH 8.0 and substrate concentration 35 mM should be done. In addition an in vivo assay shows the effect that the applications of a raw extract of the pathogen as inductor of this enzyme. The results show that the enzyme is significantly induced in carnations stems of the tolerant variety “Kiss” during the treatment by aspersion with raw extract of the pathogen while the induction was non existing for direct infection with the pathogen. The induction in this variety shows that in the pathogen extract are present elicitors for the induction of this enzyme and thus the phenylpropanoid path." @default.
- W2308985385 created "2016-06-24" @default.
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- W2308985385 date "2007-01-01" @default.
- W2308985385 modified "2023-10-06" @default.
- W2308985385 title "INDUCCIÓN DE LA ACTIVIDAD DE LA ENZIMA FENILALANINA AMONIO LIASA EN CLAVEL (Dianthus caryophyllus L) POR ELICITORES DEL HONGO Fusarium oxysporum f. sp. Dianthi raza 2 PHENYLALANINE AMMONIUM LIASE INDUCTION ON CARNATION (Dianthus caryophyllus L) BY ELICITORS FROM Fusarium oxysporum f. sp. Dianthi race 2 INDUÇÃO DA ACTIVIDADE FENILALANINA AMONIO LIASA EM CRAVO (Dianthus caryophyllus L) POR ELICITORES DO FUNGO Fusarium oxysporum f. sp. Dianthi raça 2" @default.
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- W2308985385 hasPublicationYear "2007" @default.
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