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- W230906454 abstract "Enterococcus faecium and Lactobacillus rhamnosus strains (type and reference strains, commercial strains with probiotic functionality) were studied regarding to their genetic identity using protein and DNA fingerprinting techniques. Polymerase chain reaction (PCR) using species-specific primers previously reported by other authors was shown to be a reliable tool for detecting and identifying both species. Pulsed-field gel electrophoresis (PFGE) using several rare-cutting restriction enzymes (e.g., ApaI, NotI, SmaI, XhoI) revealed reproducible strain-specific patterns and proved to be the most reliable and highly discriminating typing method. Randomly amplified polymorphic DNA (RAPD) analysis was shown to be a low-budget, rapid and simple PCR-based typing technique. However, using 57 different arbitrary primers, RAPD patterns showed a discriminatory power as high as it was observed for PFGE restriction patterns. Thus, RAPD-PCR has the potential to be developed as a routine method for the identity control and authentication of distinct LAB strains with probiotic functionality." @default.
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- W230906454 date "2003-01-01" @default.
- W230906454 modified "2023-09-26" @default.
- W230906454 title "Identification of lactic acid bacterial strains using molecular biology methods" @default.
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