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• W2309927306 abstract "The effect of phenylalanine removal on the peptide profile of the protein hydrolysates from wheat flour was investigated. Nine hydrolysates were prepared, using a successive association of a pancreatin and a crude enzymatic extract obtained from pineapple peel (CE), and the effect of the order of enzymes addition, the reaction temperature, the enzyme: substrate (E:S) ratio, and the physical treatment of sample were examined. The analysis of peptide profile of hydrolysates was performed in two stages, that is before and after removing phenylalanine. The size-exclusion high performance liquid chromatography was used for performing the fractionation, followed by Correct Fraction Area technique for quantifying peptides and free amino acids. The process of phenylalanine removal improved the peptide profile of three hydrolyzed samples, but it did not affect the five hydrolysates. The beneficial effect of this process is correlated with the increase of di- and tripeptides contents, or in reducing the amount of large peptides. The best peptide profile was obtained after phenylalanine removal by using pancreatin at E:S ratio of 4:100 at 50oC for 210min, followed by CE at E:S ratio of 10:100 at 70oC for 90 min." @default.
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• W2309927306 date "2010-01-01" @default.
• W2309927306 modified "2023-09-24" @default.
• W2309927306 title "Efeito da remoção de fenilalanina sobre o perfil peptídico de hidrolisados proteicos da farinha de trigo Phenylalanine removal effect on the peptide profile of hydrolyzed protein from wheat flour" @default.
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